Separation of derivatized carbohydrates by capillary zone electrophoresis

NWIPB OpenIR

	Separation of derivatized carbohydrates by capillary zone electrophoresis
	Sheng Xiao 1; Ding Chen-Xu 2; Liu Ling-Jun 1; Soo You-Rui 2; Sun Zhi-Wei 1; You Jin-Mao 1,2
	2008-03-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	36 期号:3 页码:280-284
文章类型	Article
摘要	A simple and rapid method was developed for the separation of derivatized carbohydrates using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as derivatization reagent by capillary zone electrophoresis. The detection was performed with diode array at 254 nm. A 58.5 cm x 50 mu m i.d. ( 50 cm effective length) untreated fused-silica capillary was used. To optimize the conditions, the background electrolyte concentration, buffer pH, column temperature, voltage and other factors were studied. It was found that the buffer concentration and pH value have a greater impact on the separation, but the influences of temperature, voltage and additives on resolution were not obvious. On the basis of the optimization, the optimum conditions are as follows: 55 mmol/L borate buffer (pH 9.46), voltage 22 kV, column temperature at 20 degrees C and detection at 254 nm. The samples were atmospherically introduced with an injection time of 10 s. The results indicate that 9 NMP-derivatized carbohydrates achieve baseline resolution within 18 min under the proposed conditions. The Tangufican Nitratia sample was also analyzed under the optimum conditions.
关键词	Carbohydrates Capillary Zone Electrophoresis 1-naphthyl-3-methyl-5-pyrazolone
WOS标题词	Science & Technology ; Physical Sciences
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000254846900002
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6880
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,et al. Separation of derivatized carbohydrates by capillary zone electrophoresis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(3):280-284.
APA	Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,Soo You-Rui,Sun Zhi-Wei,&You Jin-Mao.(2008).Separation of derivatized carbohydrates by capillary zone electrophoresis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(3),280-284.
MLA	Sheng Xiao,et al."Separation of derivatized carbohydrates by capillary zone electrophoresis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.3(2008):280-284.