Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers

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	Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers
	Li, Xiaojuan 1; Yang, Huiling 1,2; Liu, Jianquan 1
	2008
发表期刊	BELGIAN JOURNAL OF BOTANY
卷号	141 期号:1 页码:95-102
文章类型	Article
摘要	Gentiana straminea Maxim. is an outcrossing perennial from the Qinghai-Tibetan Plateau (QTP) that undergoes some selfing. This species has become threatened in recent years because of over-collection for medicinal use. A complete understanding of the partitioning of genetic variation between populations is needed to establish effective conservation measures for this species. In this study, we used RAPDs to quantify the genetic diversity of seven populations in the core distribution of G. straminea. Of the 78 primers screened, 15 produced highly reproducible bands. Using these primers, 127 discernible DNA fragments were generated, of which 110 (86.61%) were polymorphic, indicating considerable genetic variation at the species level. However, there were relatively low levels of polymorphism at the population level, with the percentage of polymorphic bands and the average gene diversity ranging from 43.31% to 51.18% and from 0.175 to 0.200, respectively. The correlation between gene diversity and population size was only marginally significant. High genetic differentiation was found between populations (Nei's genetic diversity analysis: G(ST) = 0.408; AMOVA analysis: Phi(ST) = 0.413). These differentiation measures were higher than that expected for an outbreeding species and the higher level of genetic partitioning between G. straminea populations may be a result of the combined effects of geitonogamous selfing, complex geography and past climatic oscillations. The high genetic differentiation detected between populations suggests that as many populations as possible should be included in any in situ or ex situ conservation program for this species.
关键词	Gentiana Straminea Genetic Diversity Population Differentiation Rapd
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SOUTH-AMERICAN CONIFER ; SEED PRODUCTION ; SCABIOSA-COLUMBARIA ; ARBITRARY PRIMERS ; CENTRAL GERMANY ; SIZE ; DIVERSITY ; PLANT ; DIFFERENTIATION ; CONSERVATION
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000259389100008
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6882
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW 1nstitute Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaojuan,Yang, Huiling,Liu, Jianquan. Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers[J]. BELGIAN JOURNAL OF BOTANY,2008,141(1):95-102.
APA	Li, Xiaojuan,Yang, Huiling,&Liu, Jianquan.(2008).Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers.BELGIAN JOURNAL OF BOTANY,141(1),95-102.
MLA	Li, Xiaojuan,et al."Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers".BELGIAN JOURNAL OF BOTANY 141.1(2008):95-102.