Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry

NWIPB OpenIR

	Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry
	Sun Xue-Jun 1; Sun Zhi-Wei 2; Hu Bao-Jun 1; Sheng Xiao 1; You Jin-Mao 1,2
	2008-10-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	36 期号:10 页码:1309-1315
文章类型	Article
摘要	Studies on the MS cleavage information of mono-carbohydrate derivatives using the labeling reagent 1-(2-naphthyl) -3-methyl-5-pyrazolone (NMP) coupled with liquid chromatography/electrospray ionization mass spectrometry(LC-ESI-MS) are reported. Carbohydrate derivatives show sufficient ionization information under ESI-MS in positive-ion mode. An intense protonated molecular ion at m/z [M + H](+) and characteristic fragment ion at m/z 473.0 can be used for the accurately qualitative analysis of carbohydrates. No interferences for all aliphatic and aromatic aldehydes presented in natural environmental samples were observed clue to the highly specific parent mass-to-charge ratio and the characteristic fragment ions. The results from ESI-MS experiments, especially involving the characteristic fragment ions for the accurately qualitative analysis of carbohydrate derivatives, were emphasized. The carbohydrates from the hydrolyzed rape bee pollen sample including mannose, galacturonic acid, glucuronic acid, rhamnose, glucose, galactose, xylose, arabinose and fucose can successfully be identified. It will be possible to develop a promising ESI-MS technique for carbohydrate analysis. The developed method for the identification of carbohydrate compositions is very accurate and reliable.
关键词	High Performance Liquid Chromatography-mass Spectrometry Pre-column Derivatization Monosaccharides 1-(2-naphthyl)-3-methyl-5-pyrazolone
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; 1-PHENYL-3-METHYL-5-PYRAZOLONE DERIVATIVES ; OLIGOSACCHARIDES
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000260694900001
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6884
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Ogan Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Sun Xue-Jun,Sun Zhi-Wei,Hu Bao-Jun,et al. Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(10):1309-1315.
APA	Sun Xue-Jun,Sun Zhi-Wei,Hu Bao-Jun,Sheng Xiao,&You Jin-Mao.(2008).Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(10),1309-1315.
MLA	Sun Xue-Jun,et al."Cleavage Information of Mono-Saccharide Derivatives with Electrospray Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.10(2008):1309-1315.