Extraction of pollen lipids by SFE-CO2 and determination of free fatty acids by HPLC

NWIPB OpenIR

	Extraction of pollen lipids by SFE-CO2 and determination of free fatty acids by HPLC
	Wang, Xiaoyan 1,2; Wang, Honglun 1; Liu, Yongjun 1; You, Jinmao 1; Suo, Yourui 1
	2009-02-01
发表期刊	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
卷号	111 期号:2 页码:155-163
文章类型	Review
摘要	Rape bee pollen lipids obtained by petrol ether extraction (PEE) or supercritical fluid (carbon dioxide) extraction (SFE) were compared with regard to their free fatty acid (FFA) components. Optimal SFE conditions were selected by carrying out the Taguchi method with an OA9 (3(3)) matrix design, and are as follows: extraction pressure at 35 MPa, temperature at 45 degrees C, and dynamic extraction time at 90 min. The lipid yield based on PEE was 7.42 wt-% and the extracts of the desired analytes based on SFE varied in the range of 3.23-5.58 wt-% under different conditions. With the optimized procedure, the lipid yield was 6.09 wt-%. The FFA in the lipids were separated with a pre-column derivation method and 1-[2-(p-toluensulfonate) ethyl]-2-phenylimidazole [4,5-f]9, 10-phenanthrene as labeling regent, followed by high-pressure liquid chromatography (HPLC) with fluorescence detection. analysis shows that the lipids contain abundant unsaturated fatty acids (UFA) in high to low concentrations as follows: linolenic acid (18:3), oleic acid (18:1), linoleic acid (18:2), nervonic acid (24:1), and lignoceric acid (20:4). The UFA contents in the SFE. extracts were higher than those after PEE. The results indicated that SFE under suitable conditions is more selective than conventional PEE with regard to lipid extraction and preservation of their quality.
关键词	Free Fatty Acids Hplc-fld Petrol Ether Extraction Rape Bee Pollen Lipid Supercritical Co2 Extraction
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; CHRONIC PROSTATITIS ; OIL ; IDENTIFICATION ; GOATS
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology ; Nutrition & Dietetics
WOS类目	Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000264009400007
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6886
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoyan,Wang, Honglun,Liu, Yongjun,et al. Extraction of pollen lipids by SFE-CO2 and determination of free fatty acids by HPLC[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2009,111(2):155-163.
APA	Wang, Xiaoyan,Wang, Honglun,Liu, Yongjun,You, Jinmao,&Suo, Yourui.(2009).Extraction of pollen lipids by SFE-CO2 and determination of free fatty acids by HPLC.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,111(2),155-163.
MLA	Wang, Xiaoyan,et al."Extraction of pollen lipids by SFE-CO2 and determination of free fatty acids by HPLC".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 111.2(2009):155-163.