中国科学院西北高原生物研究所机构知识库
Advanced  
NWIPB OpenIR  > 中国科学院西北高原生物研究所  > 期刊论文
题名: The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus
作者: Jiang, Yuanzhong1, 2; Guo, Li1; Ma, Xiaodong3, 4; Zhao, Xin1; Jiao, Bo1; Li, Chaofeng1, 3; Luo, Keming1
刊名: TREE PHYSIOLOGY
出版日期: 2017-05-01
卷号: 37, 期号:5, 页码:665-675
关键词: CRISPER ; Cas9 ; fungal pathogen ; plant defense ; Populus ; WRKY transcription factor
DOI: 10.1093/treephys/tpx008
文章类型: Article
英文摘要: WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Forestry
研究领域[WOS]: Forestry
关键词[WOS]: MULTIPLE SEQUENCE ALIGNMENT ; PLANT-DISEASE RESISTANCE ; SALICYLIC-ACID ; ARABIDOPSIS-THALIANA ; ACTIVE OXYGEN ; DEFENSE ; EXPRESSION ; IMMUNITY ; TOLERANCE ; SYNTHASE
收录类别: SCI
项目资助者: National Key Research and Development Program(2016YFD0600105) ; Open Fund of State Key Laboratory of Forest Genetics and Tree Breeding(TGB2015004) ; Chinese Academy of Forestry, China ; National Natural Science Foundation of China(31370672 ; 31300990)
语种: 英语
WOS记录号: WOS:000402617800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://210.75.249.4/handle/363003/7019
Appears in Collections:中国科学院西北高原生物研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing Key Lab Transgen Plant & Safety Control, 1 Tiansheng Rd, Chongqing 400715, Peoples R China
2.Sichuan Univ, MOE Key Lab Bioresources & Ecoenvironm, Coll Life Sci, 24,South Sect 1,Yihuan Rd, Chengdu 610065, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China
4.Qinghai Univ Nationalities, Sch Chem & Chem Engn, 3 Bayi Mid Rd, Xining 810007, Qinghai, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jiang, Yuanzhong]'s Articles
[Guo, Li]'s Articles
[Ma, Xiaodong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jiang, Yuanzhong]‘s Articles
[Guo, Li]‘s Articles
[Ma, Xiaodong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!