The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus

doi:10.1093/treephys/tpx008

NWIPB OpenIR

	The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus
	Jiang, Yuanzhong 1,2; Guo, Li 1; Ma, Xiaodong 3,4; Zhao, Xin 1; Jiao, Bo 1; Li, Chaofeng 1,3; Luo, Keming 1
	2017-05-01
发表期刊	TREE PHYSIOLOGY
卷号	37 期号:5 页码:665-675
文章类型	Article
摘要	WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.
关键词	Crisper Cas9 Fungal Pathogen Plant Defense Populus Wrky Transcription Factor
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1093/treephys/tpx008
关键词[WOS]	MULTIPLE SEQUENCE ALIGNMENT ; PLANT-DISEASE RESISTANCE ; SALICYLIC-ACID ; ARABIDOPSIS-THALIANA ; ACTIVE OXYGEN ; DEFENSE ; EXPRESSION ; IMMUNITY ; TOLERANCE ; SYNTHASE
收录类别	SCI
语种	英语
项目资助者	National Key Research and Development Program(2016YFD0600105) ; Open Fund of State Key Laboratory of Forest Genetics and Tree Breeding(TGB2015004) ; Chinese Academy of Forestry, China ; National Natural Science Foundation of China(31370672 ; 31300990)
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000402617800011
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7019
专题	中国科学院西北高原生物研究所
作者单位	1.Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing Key Lab Transgen Plant & Safety Control, 1 Tiansheng Rd, Chongqing 400715, Peoples R China 2.Sichuan Univ, MOE Key Lab Bioresources & Ecoenvironm, Coll Life Sci, 24,South Sect 1,Yihuan Rd, Chengdu 610065, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China 4.Qinghai Univ Nationalities, Sch Chem & Chem Engn, 3 Bayi Mid Rd, Xining 810007, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yuanzhong,Guo, Li,Ma, Xiaodong,et al. The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus[J]. TREE PHYSIOLOGY,2017,37(5):665-675.
APA	Jiang, Yuanzhong.,Guo, Li.,Ma, Xiaodong.,Zhao, Xin.,Jiao, Bo.,...&Luo, Keming.(2017).The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.TREE PHYSIOLOGY,37(5),665-675.
MLA	Jiang, Yuanzhong,et al."The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus".TREE PHYSIOLOGY 37.5(2017):665-675.