Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique

doi:10.1016/j.ijantimicag.2017.02.013

NWIPB OpenIR

	Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique
	Pei, Jinjin 1; Jiang, Lei 2
	2017-07-01
发表期刊	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷号	50 期号:1 页码:41-46
文章类型	Article
摘要	Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described from A. davidianus until now. Here we describe a novel AMP (andricin 01) isolated from the mucus of A. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
关键词	Andrias Davidianus Mucus Antimicrobial Peptide Magnetic Cell Membrane Separation Escherichia Coli
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.ijantimicag.2017.02.013
关键词[WOS]	CHINESE ; CHROMATOGRAPHY ; SKINS
收录类别	SCI
语种	英语
项目资助者	Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Research Foundation of Education Bureau of Shaanxi, China(16JS021) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Science Foundation of Shaanxi University of Technology(SLGKYQD2014-2-18)
WOS研究方向	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS类目	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS记录号	WOS:000405983100007
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7060
专题	中国科学院西北高原生物研究所
作者单位	1.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhong 723001, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Pei, Jinjin,Jiang, Lei. Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique[J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,2017,50(1):41-46.
APA	Pei, Jinjin,&Jiang, Lei.(2017).Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,50(1),41-46.
MLA	Pei, Jinjin,et al."Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique".INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 50.1(2017):41-46.