NWIPB OpenIR
NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis
Wang, Yuqi1; Li, Ruihong1,2; Li, Demou1,3; Jia, Xiaomin1; Zhou, Dangwei1,4; Li, Jianyong1,5; Lyi, Sangbom M.1; Hou, Siyu1,6; Huang, Yulan1,7; Kochian, Leon V.1,8; Liu, Jiping1
2017-05-09
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume114Issue:19Pages:5047-5052
SubtypeArticle
AbstractMembers of the aquaporin (AQP) family have been suggested to transport aluminum (Al) in plants; however, the Al form transported by AQPs and the roles of AQPs in Al tolerance remain elusive. Here we report that NIP1; 2, a plasma membrane-localized member of the Arabidopsis nodulin 26-like intrinsic protein (NIP) subfamily of the AQP family, facilitates Al-malate transport from the root cell wall into the root symplasm, with subsequent Al xylem loading and root-toshoot translocation, which are critical steps in an internal Al tolerance mechanism in Arabidopsis. We found that NIP1; 2 transcripts are expressed mainly in the root tips, and that this expression is enhanced by Al but not by other metal stresses. Mutations in NIP1; 2 lead to hyperaccumulation of toxic Al3+ in the root cell wall, inhibition of root-to-shoot Al translocation, and a significant reduction in Al tolerance. NIP1; 2 facilitates the transport of Al-malate, but not Al3+ ions, in both yeast and Arabidopsis. We demonstrate that the formation of the Al-malate complex in the root tip apoplast is a prerequisite for NIP1; 2-mediated Al removal from the root cell wall, and that this requires a functional root malate exudation system mediated by the Al-activated malate transporter, ALMT1. Taken together, these findings reveal a critical linkage between the previously identified Al exclusion mechanism based on root malate release and an internal Al tolerance mechanism identified here through the coordinated function of NIP1; 2 and ALMT1, which is required for Al removal from the root cell wall, root-to-shoot Al translocation, and overall Al tolerance in Arabidopsis.
KeywordAquaporin Nodulin 26-like Protein Aluminum Tolerance Organic Acid Exudation Malate
WOS HeadingsScience & Technology
DOI10.1073/pnas.1618557114
WOS KeywordMAJOR INTRINSIC PROTEINS ; TRANSITION ZONE ; MALATE TRANSPORTER ; INDUCED INHIBITION ; ACTIVATED CITRATE ; PLANT AQUAPORINS ; WHEAT ROOTS ; ACID SOILS ; RESISTANCE ; GENES
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000400818400059
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/7065
Collection中国科学院西北高原生物研究所
Affiliation1.ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA
2.Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
3.Southwest Univ, Biotechnol Res Ctr, Chongqing 400715, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
5.Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
6.Shanxi Agr Univ, Sch Agr, Jinzhong 030801, Peoples R China
7.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China
8.Univ Saskatchewan, Global Inst Food Secur, Saskatoon, SK S7N 4J8, Canada
Recommended Citation
GB/T 7714
Wang, Yuqi,Li, Ruihong,Li, Demou,et al. NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(19):5047-5052.
APA Wang, Yuqi.,Li, Ruihong.,Li, Demou.,Jia, Xiaomin.,Zhou, Dangwei.,...&Liu, Jiping.(2017).NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(19),5047-5052.
MLA Wang, Yuqi,et al."NIP1; 2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.19(2017):5047-5052.
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