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PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar
Wan, Shuzhen1,2; Li, Chaofeng1,2,3; Ma, Xiaodong1,2,4; Luo, Keming1,2,3
2017-08-01
发表期刊PLANT CELL REPORTS
卷号36期号:8页码:1263-1276
文章类型Article
摘要R2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs involved in anthocyanin and proanthocyanidin (PA) biosynthesis have been identified in poplar. In this study, we report the identification and characterization of a subgroup 4 MYB member PtrMYB57, which contains a repressor domain (LxLxL) at the C-terminal end. PtrMYB57 encodes an R2R3 MYB protein localized in the nucleus and is predominantly expressed in mature leaves. Transgenic poplar overexpressing PtrMYB57 showed a reduction in anthocyanin and PA accumulation compared to wild-type plants. By contrast, a high anthocyanin and PA phenotype was observed in Ptrmyb57 mutants generated by the CRISPR/Cas9 system. Furthermore, transient expression assays revealed that PtrMYB57 interacted with bHLH131 (bHLH) and PtrTTG1 (WDR) to form the MBW complex and bound to the flavonoid gene promoters, leading to inhibition of these promoters. Taken together, our results suggest that PtrMYB57 plays a negative role in the regulation of anthocyanin and PA biosynthesis in poplar.
关键词Poplar Ptrmyb57 Anthocyanin Proanthoanidin Repressor Crispr/cas9
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00299-017-2151-y
关键词[WOS]R2R3-MYB TRANSCRIPTION FACTOR ; DEVELOPING GRAPE BERRIES ; GENOME-WIDE ANALYSIS ; ARABIDOPSIS-THALIANA ; PHENYLPROPANOID BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; GENE ENCODES ; DIHYDROFLAVONOL REDUCTASE ; POPULUS-TRICHOCARPA ; TRANSGENIC TOBACCO
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(31370672 ; "One Hundred Talents Program'' of the Chinese Academy of Sciences ; 31500216 ; 31500544)
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000405680300006
引用统计
被引频次:67[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/7070
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810008, Peoples R China
2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
3.Southwest Univ, Sch Life Sci, 1,Tiansheng Rd, Chongqing 400715, Peoples R China
4.Qinghai Univ Nationalities, China Sch Chem & Chem Engn, 3 Bayi Middle Rd, Xining 810007, Qinghai, Peoples R China
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GB/T 7714
Wan, Shuzhen,Li, Chaofeng,Ma, Xiaodong,et al. PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar[J]. PLANT CELL REPORTS,2017,36(8):1263-1276.
APA Wan, Shuzhen,Li, Chaofeng,Ma, Xiaodong,&Luo, Keming.(2017).PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar.PLANT CELL REPORTS,36(8),1263-1276.
MLA Wan, Shuzhen,et al."PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar".PLANT CELL REPORTS 36.8(2017):1263-1276.
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