Knowledge Management System of Northwest Institute of Plateau Biology, CAS
PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar | |
Wan, Shuzhen1,2; Li, Chaofeng1,2,3; Ma, Xiaodong1,2,4; Luo, Keming1,2,3 | |
2017-08-01 | |
发表期刊 | PLANT CELL REPORTS |
卷号 | 36期号:8页码:1263-1276 |
文章类型 | Article |
摘要 | R2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs involved in anthocyanin and proanthocyanidin (PA) biosynthesis have been identified in poplar. In this study, we report the identification and characterization of a subgroup 4 MYB member PtrMYB57, which contains a repressor domain (LxLxL) at the C-terminal end. PtrMYB57 encodes an R2R3 MYB protein localized in the nucleus and is predominantly expressed in mature leaves. Transgenic poplar overexpressing PtrMYB57 showed a reduction in anthocyanin and PA accumulation compared to wild-type plants. By contrast, a high anthocyanin and PA phenotype was observed in Ptrmyb57 mutants generated by the CRISPR/Cas9 system. Furthermore, transient expression assays revealed that PtrMYB57 interacted with bHLH131 (bHLH) and PtrTTG1 (WDR) to form the MBW complex and bound to the flavonoid gene promoters, leading to inhibition of these promoters. Taken together, our results suggest that PtrMYB57 plays a negative role in the regulation of anthocyanin and PA biosynthesis in poplar. |
关键词 | Poplar Ptrmyb57 Anthocyanin Proanthoanidin Repressor Crispr/cas9 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s00299-017-2151-y |
关键词[WOS] | R2R3-MYB TRANSCRIPTION FACTOR ; DEVELOPING GRAPE BERRIES ; GENOME-WIDE ANALYSIS ; ARABIDOPSIS-THALIANA ; PHENYLPROPANOID BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; GENE ENCODES ; DIHYDROFLAVONOL REDUCTASE ; POPULUS-TRICHOCARPA ; TRANSGENIC TOBACCO |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(31370672 ; "One Hundred Talents Program'' of the Chinese Academy of Sciences ; 31500216 ; 31500544) |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:000405680300006 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/7070 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810008, Peoples R China 2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China 3.Southwest Univ, Sch Life Sci, 1,Tiansheng Rd, Chongqing 400715, Peoples R China 4.Qinghai Univ Nationalities, China Sch Chem & Chem Engn, 3 Bayi Middle Rd, Xining 810007, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Wan, Shuzhen,Li, Chaofeng,Ma, Xiaodong,et al. PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar[J]. PLANT CELL REPORTS,2017,36(8):1263-1276. |
APA | Wan, Shuzhen,Li, Chaofeng,Ma, Xiaodong,&Luo, Keming.(2017).PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar.PLANT CELL REPORTS,36(8),1263-1276. |
MLA | Wan, Shuzhen,et al."PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar".PLANT CELL REPORTS 36.8(2017):1263-1276. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论