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Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro
Luan, Guangxiang1,2; Tie, Fangfang1,2; Yuan, Zhenzhen1,2; Li, Gang1,3; He, Jie3; Wang, Zhenhua3; Wang, Honglun1,4
2017-07-01
发表期刊CHEMISTRY & BIODIVERSITY
卷号14期号:7
文章类型Article
摘要Obesity, a major health problem worldwide, is a complex multifactorial chronic disease that increases the risk for insulin resistance, type 2 diabetes, coronary heart disease, and hypertension. In this study, we assessed methods to isolate hypaphorine, a potent drug candidate for obesity and insulin resistance. Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) was established as a method to separate three compounds, adenosine, l-tryptophan, and hypaphorine, from the crude extracts of Caragana korshinskii Kom. Due to its specific chemical structure, the effect of hypaphorine on differentiation and dexamethasone (DXM) induced insulin resistance of 3T3-L1 cells was investigated. The structures of the three compounds were confirmed by UV, H-1-NMR, and C-13-NMR analysis and compared with published data. The activity results indicated that hypaphorine prevented the differentiation of 3T3-L1 preadipocytes into adipocytes by down-regulating hormone-stimulated protein expression of peroxisome proliferator activated receptor (PPAR) and CCAAT/enhancer binding protein (C/EBP), and their downstream targets, sterol regulatory element binding protein 1 c (SREBP1c) and fatty acid synthase (FAS). Hypaphorine also alleviated DXM-induced insulin resistance in differentiated 3T3-L1 adipocytes via increasing the phosphorylation level of Akt2, a key protein in the insulin signaling pathway. Taken together, we suggest that the method can be applied to large-scale extraction and large-quantity preparation of hypaphorine for treatment of obesity and insulin resistance.
关键词Isolation Hypaphorine Adipocyte Differentiation Insulin Resistance 3t3-l1 Cells
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1002/cbdv.201700038
关键词[WOS]FUNGAL HYPAPHORINE ; ADIPOGENESIS ; PURIFICATION ; COMBINATION ; SEPARATION ; CELLS ; HSCCC ; CONSTITUENTS ; CHINENSIS ; RECEPTOR
收录类别SCI
语种英语
项目资助者National Science Foundation of China(31470426 ; Qinghai Provincial Science Foundation(2015-ZJ-728 ; Taishan Scholar Program of Shandong Province(tshw201502046) ; State Key Laboratory of Plateau Eclology and Agriculture, Qinghai Unviersity(2016-KF-05) ; 31300292) ; 2016-ZJ-01 ; 2015-NK-302)
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号WOS:000405127700009
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/7074
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, 23 Xinning Rd, Xining 810008, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yantai Univ, Ctr Mitochondria & Healthy Aging, Coll Life Sci, 30 Qingquan Rd, Yantai 264005, Peoples R China
4.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810008, Peoples R China
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GB/T 7714
Luan, Guangxiang,Tie, Fangfang,Yuan, Zhenzhen,et al. Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro[J]. CHEMISTRY & BIODIVERSITY,2017,14(7).
APA Luan, Guangxiang.,Tie, Fangfang.,Yuan, Zhenzhen.,Li, Gang.,He, Jie.,...&Wang, Honglun.(2017).Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro.CHEMISTRY & BIODIVERSITY,14(7).
MLA Luan, Guangxiang,et al."Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro".CHEMISTRY & BIODIVERSITY 14.7(2017).
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