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3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification
You, Jinmao1,2; Dou, Kun1; Song, Cuihua1; Li, Guoliang1; Sun, Zhiwei1; Zhang, Shijuan1; Chen, Guang1; Zhao, Xianen1; Hu, Na2; Zhou, Wu3
2017-06-01
发表期刊JOURNAL OF SEPARATION SCIENCE
卷号40期号:12页码:2528-2540
文章类型Article
摘要A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)-N-(9-ethyl-9H)-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50 degrees C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at. ex = 276 nm and an emission maximum at lambda(em) = 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033-6.66 mu mol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94-5.77 mu g/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries (n = 3) were in the range of 87.21-101.12%.
关键词Fluorescence Detection High-performance Liquid Chromatography Rubber Thiophenols
WOS标题词Science & Technology ; Physical Sciences
DOI10.1002/jssc.201601166
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; THIOL-CONTAINING COMPOUNDS ; LIVING CELLS ; WATER SAMPLES ; SENSITIVE DETECTION ; BIOLOGICAL SAMPLES ; HUMAN PLASMA ; CYSTEINE ; HOMOCYSTEINE ; GLUTATHIONE
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21475075 ; Natural Science Foundation of Shandong Province(ZR2015CM040 ; 21305076 ; ZR2014BQ009) ; 21275089)
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000404027800001
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/7083
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
3.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China
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GB/T 7714
You, Jinmao,Dou, Kun,Song, Cuihua,et al. 3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(12):2528-2540.
APA You, Jinmao.,Dou, Kun.,Song, Cuihua.,Li, Guoliang.,Sun, Zhiwei.,...&Zhou, Wu.(2017).3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.JOURNAL OF SEPARATION SCIENCE,40(12),2528-2540.
MLA You, Jinmao,et al."3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification".JOURNAL OF SEPARATION SCIENCE 40.12(2017):2528-2540.
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