NWIPB OpenIR
A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry
Zheng, Longfang1,2; Zhao, Xian-En1,2,3,4; Zhu, Shuyun1,2; Tao, Yanduo4; Ji, Wenhua3; Geng, Yanling3; Wang, Xiao3; Chen, Guang1,2; You, Jinmao1,2,4
2017-06-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume1054Pages:64-72
SubtypeArticle
AbstractIn this work, for the first time, a new hyphenated technique of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction has been developed for the simultaneous determination of monoamine neurotransmitters (MANTs) and their biosynthesis precursors and metabolites. The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry detection using multiple-reaction monitoring mode. A pair of mass spectrometry sensitizing reagents, d(0)-10-methyl-acridone-2-sulfonyl chloride and d(3)-10-methyl-acridone-2-sulfonyl chloride, as stable isotope probes was utilized to facilely label neurotransmitters, respectively. The heavy labeled MANTs standards were prepared and used as internal standards for quantification to minimize the matrix effects in mass spectrometry analysis. Low toxic bromobenzene (extractant) and acetonitrile (dispersant) were utilized in microextraction procedure. Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N > 10) in the range of 0.002-0.010 and 0.015-0.040 nmol/L, respectively. Meanwhile, it also brought acceptable precision (4.2-8.8%, peak area RSDs %) and accuracy (recovery, 96.9-104.1%) results. This method was successfully applied to the simultaneous determination of monoamine neurotransmitters and their biosynthesis precursors and metabolites in rat brain microdialysates of Parkinson's disease and normal rats. This provided a new method for the neurotransmitters related studies in the future.
KeywordIn Vivo Microdialysis Neurotransmitters Triple Quadrupole Mass Spectrometry Sensitivity Enhancement Stable Isotope-labeling Derivatization Targeted Metabolomics
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2017.03.039
WOS KeywordSOLID-PHASE EXTRACTION ; MONOAMINE NEUROTRANSMITTERS ; UHPLC-MS/MS ; AMINO-ACID ; LC-MS/MS ; CATECHOLAMINE NEUROTRANSMITTERS ; CHEMILUMINESCENCE DETECTION ; SIMULTANEOUS QUANTIFICATION ; MULTIPLE NEUROTRANSMITTERS ; CAPILLARY-ELECTROPHORESIS
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21405094 ; Key Laboratory of Tibetan Medicine Research (Chinese Academy of Sciences) ; Key Laboratory of TCM Quality Control Technology(2016003) ; Foundation of Qufu Normal University(BSQD2012019 ; Qufu Normal University(sj201402) ; 81603286 ; BSQD2012023) ; 81303179 ; 21475075)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000402219000009
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/7091
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Longfang,Zhao, Xian-En,Zhu, Shuyun,et al. A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1054:64-72.
APA Zheng, Longfang.,Zhao, Xian-En.,Zhu, Shuyun.,Tao, Yanduo.,Ji, Wenhua.,...&You, Jinmao.(2017).A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1054,64-72.
MLA Zheng, Longfang,et al."A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1054(2017):64-72.
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