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题名: A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry
作者: Zheng, Longfang1, 2; Zhao, Xian-En1, 2, 3, 4; Zhu, Shuyun1, 2; Tao, Yanduo4; Ji, Wenhua3; Geng, Yanling3; Wang, Xiao3; Chen, Guang1, 2; You, Jinmao1, 2, 4
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期: 2017-06-01
卷号: 1054, 页码:64-72
关键词: In vivo microdialysis ; Neurotransmitters ; Triple quadrupole mass spectrometry ; Sensitivity enhancement ; Stable isotope-labeling derivatization ; Targeted metabolomics
DOI: 10.1016/j.jchromb.2017.03.039
文章类型: Article
英文摘要: In this work, for the first time, a new hyphenated technique of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction has been developed for the simultaneous determination of monoamine neurotransmitters (MANTs) and their biosynthesis precursors and metabolites. The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry detection using multiple-reaction monitoring mode. A pair of mass spectrometry sensitizing reagents, d(0)-10-methyl-acridone-2-sulfonyl chloride and d(3)-10-methyl-acridone-2-sulfonyl chloride, as stable isotope probes was utilized to facilely label neurotransmitters, respectively. The heavy labeled MANTs standards were prepared and used as internal standards for quantification to minimize the matrix effects in mass spectrometry analysis. Low toxic bromobenzene (extractant) and acetonitrile (dispersant) were utilized in microextraction procedure. Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N > 10) in the range of 0.002-0.010 and 0.015-0.040 nmol/L, respectively. Meanwhile, it also brought acceptable precision (4.2-8.8%, peak area RSDs %) and accuracy (recovery, 96.9-104.1%) results. This method was successfully applied to the simultaneous determination of monoamine neurotransmitters and their biosynthesis precursors and metabolites in rat brain microdialysates of Parkinson's disease and normal rats. This provided a new method for the neurotransmitters related studies in the future.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: SOLID-PHASE EXTRACTION ; MONOAMINE NEUROTRANSMITTERS ; UHPLC-MS/MS ; AMINO-ACID ; LC-MS/MS ; CATECHOLAMINE NEUROTRANSMITTERS ; CHEMILUMINESCENCE DETECTION ; SIMULTANEOUS QUANTIFICATION ; MULTIPLE NEUROTRANSMITTERS ; CAPILLARY-ELECTROPHORESIS
收录类别: SCI
项目资助者: National Natural Science Foundation of China(21405094 ; Key Laboratory of Tibetan Medicine Research (Chinese Academy of Sciences) ; Key Laboratory of TCM Quality Control Technology(2016003) ; Foundation of Qufu Normal University(BSQD2012019 ; Qufu Normal University(sj201402) ; 81603286 ; BSQD2012023) ; 81303179 ; 21475075)
语种: 英语
WOS记录号: WOS:000402219000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://210.75.249.4/handle/363003/7091
Appears in Collections:中国科学院西北高原生物研究所_期刊论文

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作者单位: 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
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