A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry

doi:10.1016/j.jchromb.2017.03.039

NWIPB OpenIR

	A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry
	Zheng, Longfang 1,2; Zhao, Xian-En 1,2,3,4; Zhu, Shuyun 1,2; Tao, Yanduo 4; Ji, Wenhua 3; Geng, Yanling 3; Wang, Xiao 3; Chen, Guang 1,2; You, Jinmao 1,2,4
	2017-06-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷号	1054 页码:64-72
文章类型	Article
摘要	In this work, for the first time, a new hyphenated technique of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction has been developed for the simultaneous determination of monoamine neurotransmitters (MANTs) and their biosynthesis precursors and metabolites. The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry detection using multiple-reaction monitoring mode. A pair of mass spectrometry sensitizing reagents, d(0)-10-methyl-acridone-2-sulfonyl chloride and d(3)-10-methyl-acridone-2-sulfonyl chloride, as stable isotope probes was utilized to facilely label neurotransmitters, respectively. The heavy labeled MANTs standards were prepared and used as internal standards for quantification to minimize the matrix effects in mass spectrometry analysis. Low toxic bromobenzene (extractant) and acetonitrile (dispersant) were utilized in microextraction procedure. Under the optimized conditions, good linearity was observed with the limits of detection (S/N > 3) and limits of quantification (S/N > 10) in the range of 0.002-0.010 and 0.015-0.040 nmol/L, respectively. Meanwhile, it also brought acceptable precision (4.2-8.8%, peak area RSDs %) and accuracy (recovery, 96.9-104.1%) results. This method was successfully applied to the simultaneous determination of monoamine neurotransmitters and their biosynthesis precursors and metabolites in rat brain microdialysates of Parkinson's disease and normal rats. This provided a new method for the neurotransmitters related studies in the future.
关键词	In Vivo Microdialysis Neurotransmitters Triple Quadrupole Mass Spectrometry Sensitivity Enhancement Stable Isotope-labeling Derivatization Targeted Metabolomics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.jchromb.2017.03.039
关键词[WOS]	SOLID-PHASE EXTRACTION ; MONOAMINE NEUROTRANSMITTERS ; UHPLC-MS/MS ; AMINO-ACID ; LC-MS/MS ; CATECHOLAMINE NEUROTRANSMITTERS ; CHEMILUMINESCENCE DETECTION ; SIMULTANEOUS QUANTIFICATION ; MULTIPLE NEUROTRANSMITTERS ; CAPILLARY-ELECTROPHORESIS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21405094 ; Key Laboratory of Tibetan Medicine Research (Chinese Academy of Sciences) ; Key Laboratory of TCM Quality Control Technology(2016003) ; Foundation of Qufu Normal University(BSQD2012019 ; Qufu Normal University(sj201402) ; 81603286 ; BSQD2012023) ; 81303179 ; 21475075)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000402219000009
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7091
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Longfang,Zhao, Xian-En,Zhu, Shuyun,et al. A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1054:64-72.
APA	Zheng, Longfang.,Zhao, Xian-En.,Zhu, Shuyun.,Tao, Yanduo.,Ji, Wenhua.,...&You, Jinmao.(2017).A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1054,64-72.
MLA	Zheng, Longfang,et al."A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1054(2017):64-72.