Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird

doi:10.1111/mec.14090

NWIPB OpenIR

	Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird
	Pan, Shengkai 1,2,3; Zhang, Tongzuo 4; Rong, Zhengqin 5; Hu, Li 1,3,6; Gu, Zhongru 1,3; Wu, Qi 1; Dong, Shanshan 7; Liu, Qiong 1,8,9; Lin, Zhenzhen 1; Deutschova, Lucia 10; Li, Xinhai 1; Dixon, Andrew 2,11,12; Bruford, Michael W.2,13; Zhan, Xiangjiang 1,2
	2017-06-01
发表期刊	MOLECULAR ECOLOGY
卷号	26 期号:11 页码:2993-3010
文章类型	Article
摘要	Low oxygen and temperature pose key physiological challenges for endotherms living on the Qinghai-Tibetan Plateau (QTP). Molecular adaptations to high-altitude living have been detected in the genomes of Tibetans, their domesticated animals and a few wild species, but the contribution of transcriptional variation to altitudinal adaptation remains to be determined. Here we studied a top QTP predator, the saker falcon, and analysed how the transcriptome has become modified to cope with the stresses of hypoxia and hypothermia. Using a hierarchical design to study saker populations inhabiting grassland, steppe/desert and highland across Eurasia, we found that the QTP population is already distinct despite having colonized the Plateau <2000years ago. Selection signals are limited at the cDNA level, but of only seventeen genes identified, three function in hypoxia and four in immune response. Our results show a significant role for RNA transcription: 50% of upregulated transcription factors were related to hypoxia responses, differentiated modules were significantly enriched for oxygen transport, and importantly, divergent EPAS1 functional variants with a refined co-expression network were identified. Conservative gene expression and relaxed immune gene variation may further reflect adaptation to hypothermia. Our results exemplify synergistic responses between DNA polymorphism and RNA expression diversity in coping with common stresses, underpinning the successful rapid colonization of a top predator onto the QTP. Importantly, molecular mechanisms underpinning highland adaptation involve relatively few genes, but are nonetheless more complex than previously thought and involve fine-tuned transcriptional responses and genomic adaptation.
关键词	Adaptation Colonization Population Transcriptome Qinghai-tibetan Plateau Rna Sequencing Saker Falcon
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1111/mec.14090
关键词[WOS]	PRINCIPAL COMPONENT ANALYSIS ; INDUCIBLE FACTOR-I ; HIGH-ALTITUDE ; DIFFERENTIAL EXPRESSION ; GENOME SCANS ; IMMUNE-RESPONSE ; R PACKAGE ; SEQ DATA ; HYPOXIA ; ADAPTATION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31522052) ; National Key Programme of Research and Development, Ministry of Science and Technology(2016YFC0503200) ; Breakthrough Project of Strategic Priority Program of the Chinese Academy of Sciences(XDB13000000) ; Recruitment Program of Global Youth Experts of China ; Royal Society Newton Advanced Fellowship ; Environment Agency - Abu Dhabi
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号	WOS:000402040000013
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7094
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, Beichen West Rd, Beijing 100101, Peoples R China 2.Cardiff Univ, Inst Zool, Joint Lab Biocomplex Res, Beichen West Rd, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 5.Beijing Honortech Co Ltd, Beijing 102200, Peoples R China 6.BGI Shenzhen, Shenzhen 518083, Peoples R China 7.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Minist Educ, Key Lab Biomed Informat Engn, Xian 710049, Peoples R China 8.Beijing Normal Univ, Coll Life Sci, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China 9.Beijing Normal Univ, Coll Life Sci, MOE Key Lab Biodivers Sci & Ecol Engn, Beijing 100875, Peoples R China 10.Raptor Protect Slovakia, Kuklovska 5, SK-84104 Bratislava 4, Slovakia 11.Int Wildlife Consultants Ltd, POB 19, Carmarthen SA33 5YL, Dyfed, Wales 12.Environm Agcy Abu Dhabi, POB 45553,Al Mamoura Bldg A,Muroor Rd, Abu Dhabi, U Arab Emirates 13.Cardiff Univ, Cardiff Sch Biosci, Organisms & Environm Div, Cardiff CF10 3AX, S Glam, Wales
推荐引用方式 GB/T 7714	Pan, Shengkai,Zhang, Tongzuo,Rong, Zhengqin,et al. Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird[J]. MOLECULAR ECOLOGY,2017,26(11):2993-3010.
APA	Pan, Shengkai.,Zhang, Tongzuo.,Rong, Zhengqin.,Hu, Li.,Gu, Zhongru.,...&Zhan, Xiangjiang.(2017).Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird.MOLECULAR ECOLOGY,26(11),2993-3010.
MLA	Pan, Shengkai,et al."Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird".MOLECULAR ECOLOGY 26.11(2017):2993-3010.