Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology

doi:10.1002/jssc.201700144

NWIPB OpenIR

	Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology
	Cui, Yulei 1,2,3,4; Shen, Na 1,2,3,4; Dang, Jun 1,2,3; Mei, Lijuan 1,2,3; Tao, Yanduo 1,2,3; Liu, Zenggen1,2,3
	2017-07-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	40 期号:14 页码:2895-2905
文章类型	Article
摘要	Bioactive equivalent combinatorial components play a critical role in herbal medicines. However, how to discover and enrich them efficiently is a question for herbal pharmaceuticals researchers. In our work, a novel two-dimensional reversed-phase/hydrophilic interaction high-performance liquid chromatography method was established to perform real-time components trapping and combining for preparation and isolation of coeluting components. Arenaria kansuensis was taken as an example, and solid-phase extraction coupled with liquid-liquid extraction as a simple and efficient method for enriching trace components, reversed phase column coupled with hydrophilic interaction liquid chromatography XAmide column as two-dimensional chromatography technology for isolation and preparation of coeluting constituents, enzyme-linked immune-sorbent assay as bio-guided assay, and anti-inflammatory bioactivity evaluation for bioactive constituents. A combination of 12 beta-carboline alkaloidswas identified as anti-inflammatory bioactive equivalent combinatorial components from A. kansuensis, which accounts for 1.9% w/w of original A. kansuensis. This work answers the key question of which are real anti-inflammatory components from A. kansuensis and provides a fast and efficient approach for discovering and enriching trace beta-carboline alkaloids from herbal medicines for the first time. More importantly, the discovery of bioactive equivalent combinatorial components could improve the quality control of herbal products and inspire a herbal medicine based on combinatorial therapeutics.
关键词	Arenaria Kansuensis Bioactive Equivalence Of Combinatorial Components Liquid-liquid Extraction Solid-phase Extraction Two-dimensional Chromatography
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201700144
关键词[WOS]	PICRASMA-QUASSIOIDES ; MEDICINE
收录类别	SCI
语种	英语
项目资助者	West Light Foundation of The Chinese Academy of Sciences for Doctors ; Natural Science Foundation of Qinghai Province(2015-ZJ-927Q) ; Science and Technology Major Project of Qinghai Province, China(2014-GX-A3A) ; Strategic network planning project of biological resources service from Chinese academy of science(ZSTH-027) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A-01)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000406244500004
出版者	WILEY-V C H VERLAG GMBH
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9794
专题	中国科学院西北高原生物研究所
通讯作者	Tao, Yanduo; Liu, Zenggen
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 2.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 3.Key Lab Tibetan Med Res Qinghai Prov, Xining, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Yulei,Shen, Na,Dang, Jun,et al. Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(14):2895-2905.
APA	Cui, Yulei,Shen, Na,Dang, Jun,Mei, Lijuan,Tao, Yanduo,&Liu, Zenggen.(2017).Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology.JOURNAL OF SEPARATION SCIENCE,40(14),2895-2905.
MLA	Cui, Yulei,et al."Anti-inflammatory bioactive equivalence of combinatorial components beta-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology".JOURNAL OF SEPARATION SCIENCE 40.14(2017):2895-2905.