TaMYB3, Encoding a Functional MYB Transcriptor, Isolated from the Purple Pericarp of Triticum aestivum

doi:10.1556/0806.45.2017.027

NWIPB OpenIR

	TaMYB3, Encoding a Functional MYB Transcriptor, Isolated from the Purple Pericarp of Triticum aestivum
	Li, N.1,2,3; Zong, Y.1,3; Liu, B. L.1,4; Chen, W. J.1,4; Zhang, B.1,4
	2017-09-01
发表期刊	CEREAL RESEARCH COMMUNICATIONS
ISSN	0133-3720
卷号	45 期号:3 页码:369-380
文章类型	Article
摘要	Purple pericarp is an interesting and useful trait in Triticum aestivum, but the molecular mechanism behind this phenotype remains unclear. The allelic variation in the MYB transcriptors is associated with the phenotype of pigmented organs in many plants. In this study, a MYB transcription factor gene, TaMYB3, was isolated using homology-based cloning and a differentially expressed gene mining approach, to verify the function of the MYB transcriptor in the purple pericarp. The coding sequence of TaMYB3 in cultivar Gy115 was the same as that in cultivar Opata. TaMYB3 was localized to FL0.62-0.95 on chromosome 4BL. The TaMYB3 protein contains DNA-binding and transcription-activation domains, and clustered on a phylogenetic tree with the MYB proteins that regulates anthocyanin and proanthocyanin biosynthesis. TaMYB3 localized in the nuclei of Arabidopsis thaliana and wheat protoplasts after it was transiently expressed with PEG transformation. TaMYB3 induced anthocyanin synthesis in the pericarp cells of Opata in the dark in collaboration with the basic helix-loop-helix protein ZmR, which is also the function of ZmC1. However, TaMYB3 alone did not induce anthocyanin biosynthesis in the pericarp cells of the white grain wheat cultivar Opata in the light after bombardment, whereas the single protein ZmR did. Light increased the expression of TaMYB3 in the pericarp of Gy115 and Opata, but only induced anthocyanin biosynthesis in the grains of Gy115. Our results extend our understanding of the molecular mechanism of the purple pericarp trait in T. aestivum.
关键词	Myb Transcriptor Purple Pericarp Anthocyanin Biosynthesis Triticum Aestivum
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1556/0806.45.2017.027
关键词[WOS]	ANTHOCYANIN BIOSYNTHESIS ; MOLECULAR ANALYSIS ; GENE-EXPRESSION ; FLOWER COLOR ; MAIZE C1 ; WHEAT ; ARABIDOPSIS ; LOCUS ; IDENTIFICATION ; PIGMENTATION
收录类别	SCI
语种	英语
项目资助者	West Light Foundation of the Chinese Academy of Sciences ; Basic Research Projects of Qinghai Province(2015-ZJ-701) ; Chinese Academy of Sciences
WOS研究方向	Agriculture
WOS类目	Agronomy
WOS记录号	WOS:000407673200002
出版者	AKADEMIAI KIADO RT
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9795
专题	中国科学院西北高原生物研究所
通讯作者	Liu, B. L.
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 2.Qinghai Univ, Xining 810014, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Li, N.,Zong, Y.,Liu, B. L.,et al. TaMYB3, Encoding a Functional MYB Transcriptor, Isolated from the Purple Pericarp of Triticum aestivum[J]. CEREAL RESEARCH COMMUNICATIONS,2017,45(3):369-380.
APA	Li, N.,Zong, Y.,Liu, B. L.,Chen, W. J.,&Zhang, B..(2017).TaMYB3, Encoding a Functional MYB Transcriptor, Isolated from the Purple Pericarp of Triticum aestivum.CEREAL RESEARCH COMMUNICATIONS,45(3),369-380.
MLA	Li, N.,et al."TaMYB3, Encoding a Functional MYB Transcriptor, Isolated from the Purple Pericarp of Triticum aestivum".CEREAL RESEARCH COMMUNICATIONS 45.3(2017):369-380.