Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim

doi:10.1002/ptr.5875

NWIPB OpenIR

	Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim
	Yuan Xiang 1,2; Zhao Jing 3; Wang Haixia 1; Yu Ruitao 1; Wen Huaixiu 1,2; Liu Zenggen1 ; Mei Lijuan 1; Wang Yiping 3; Tao Yanduo 1
	2017-10-01
发表期刊	PHYTOTHERAPY RESEARCH
ISSN	0951-418X
卷号	31 期号:10 页码:1509-1520
文章类型	Article
摘要	The aim of the present study was to evaluate the antiproliferative effect of phenylpropanoids isolated from the n-BuOH-soluble fraction of an ethanolic extract of Lagotis brevituba Maxim. The phenylpropanoids were identified as echinacoside, lagotioside, glucopyranosyl(1-6)martynoside, plantamoside, and verbascoside. Three of the compounds, lagotioside, glucopyranosyl(1-6)martynoside, and plantamoside, were isolated from L. brevituba for the first time. The antiproliferative activity of the isolates was evaluated in human gastric carcinoma (MGC-803), human colorectal carcinoma (HCT116), human hepatocellar carcinoma (HepG2), and human lung cancer (HCT116) cells using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Plantamoside showed promising activity against MGC-803 cells, with a half maximal inhibitory concentration value of 37.09 mu M. The mechanism of the pro-apoptosis effect of plantamoside was then evaluated in MGC-803 cells. Changes in cell morphology, including disorganization of the architecture of actin microfilaments and formation of apoptotic bodies, together with cell cycle arrest in G2/M phases, were observed after treatment of plantamoside. The antiproliferative and pro-apoptotic effects were associated with a decrease in the ratio of Bcl-2/Bax and reduced mitochondrial membrane potential, which was accompanied by the release of reactive oxygen species and Ca2+ into the cytoplasm. Taken together, the results indicated that plantamoside promotes apoptosis via a mitochondria-dependent mechanism. Copyright (c) 2017 John Wiley & Sons, Ltd.
关键词	Phenylpropanoids Lagotis Brevituba Maxim Antiproliferative Plantamoside
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/ptr.5875
关键词[WOS]	APOPTOSIS ; CELLS ; MITOCHONDRIA ; GLYCOSIDES ; CANCER ; FAMILY ; DEATH
收录类别	SCI
语种	英语
项目资助者	Chinese Academy of Sciences(ZSTH-027) ; Major Science and Technology Projects in Qinghai Province(2014-GX-A3A-01) ; Qinghai Association for Science and Technology(2017KX05)
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000412164200005
出版者	WILEY
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9807
专题	中国科学院西北高原生物研究所
通讯作者	Wang Yiping; Tao Yanduo
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100000, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yuan Xiang,Zhao Jing,Wang Haixia,et al. Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim[J]. PHYTOTHERAPY RESEARCH,2017,31(10):1509-1520.
APA	Yuan Xiang.,Zhao Jing.,Wang Haixia.,Yu Ruitao.,Wen Huaixiu.,...&Tao Yanduo.(2017).Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim.PHYTOTHERAPY RESEARCH,31(10),1509-1520.
MLA	Yuan Xiang,et al."Antiproliferative Activity of Phenylpropanoids Isolated from Lagotis brevituba Maxim".PHYTOTHERAPY RESEARCH 31.10(2017):1509-1520.