A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe

doi:10.1016/j.talanta.2017.07.070

NWIPB OpenIR

	A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe
	Lei, Cuihua 1; Zhao, Xian-En 1,2; Sun, Jing 2; Yan, Xiaolu 1; Gao, Yue 1; Gao, Han 1; Zhu, Shuyun 1,2; Wang, Hua 1
	2017-12-01
发表期刊	TALANTA
ISSN	0039-9140
卷号	175 页码:457-462
文章类型	Article
摘要	A novel colorimetric method for the detection of tyrosinase (TYR) and its inhibitor by taking utilization of Ag+-3,3 ',5,5 '-tetramethylbenzidine (TMB) detection system has been proposed. Ag+ could oxidize TMB to oxidized TMB (oxTMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm. The addition of dopamine (DA) could cause the reduction of oxTMB which resulted in the fading of the blue color and a decrease of the absorbance at 652 nm. However, in the presence of TYR, DA could be oxidized to dopaquinone, which inhibited the reduction of oxTMB by DA, resulting in a blue color recovery and an increase of the absorbance at 652 nm. Based on this finding, we propose a method to quantitatively detect TYR activity with the help of UV-vis spectroscopy. The developed assay is highly sensitive with a low detection limit of 0.010 U/mL. More importantly, this method is fairly simple and inexpensive without the use of complicated nanomaterials. In addition, it constructs a useful platform for TYR inhibitor screening.
关键词	Silver Ion (Ag++) Tyrosinase Enzyme Inhibition Colorimetric Assay 3 3 ' 5 5 '-tetramethylbenzidine (Tmb)
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.talanta.2017.07.070
关键词[WOS]	QUANTUM DOTS ; BIOCATALYTIC TRANSFORMATIONS ; MUSHROOM TYROSINASE ; GOLD NANOCLUSTERS ; DUAL-EMISSION ; IN-VITRO ; DOPAMINE ; NANOPARTICLES ; GLUTATHIONE ; CELLS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21405094 ; Qinghai Key Laboratory(2017-ZJ-Y10) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; National Undergraduate Research Training Program(201610446033) ; 81303179 ; 21675099)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000412033100060
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9810
专题	中国科学院西北高原生物研究所
通讯作者	Zhao, Xian-En; Zhu, Shuyun; Wang, Hua
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu City 273165, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Lei, Cuihua,Zhao, Xian-En,Sun, Jing,et al. A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe[J]. TALANTA,2017,175:457-462.
APA	Lei, Cuihua.,Zhao, Xian-En.,Sun, Jing.,Yan, Xiaolu.,Gao, Yue.,...&Wang, Hua.(2017).A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe.TALANTA,175,457-462.
MLA	Lei, Cuihua,et al."A simple and novel colorimetric assay for tyrosinase and inhibitor screening using 3,3 ',5,5 '-tetramethylbenzidine as a chromogenic probe".TALANTA 175(2017):457-462.