NWIPB OpenIR
A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine
Qu, Fei1,2; Liu, Yining1,2; Kong, Rongmei1,2; You, Jinmao1,2,3
2017-11-01
发表期刊MICROCHIMICA ACTA
ISSN0026-3672
卷号184期号:11页码:4417-4424
文章类型Article
摘要The authors report that the fluorescence of polymer-capped silver nanoclusters (AgNCs) is quenched by MoS2 nanosheets. However, in the presence of electrolytes such as sodium acetate (50 mM), the nanosheets tend to aggregate, and this causes fluorescence to be restored. If, however, ssDNA is added, it will be adsorbed on the surface of the MoS2 nanosheets and stabilize them against aggregation. As a result, the emission of the AgNCs (best measured at excitation/emission wavelengths of 375/455 nm) is quenched again. If complementary oligonucleotide is added, a dsDNA will be formed that does no longer prevent aggregation and fluorescence is restored. Similarly, if an aptamer is adsorbed on the MoS2 nanosheets and its substrate is then added to form a folded state, the fluorescence of the AgNCs also will be restored. As a proof of concept, the DNA sequences of hepatitis B virus and of a dopamine-selective aptamer were chosen as models. The resulting assays have low detection limits and linear ranges that extend from 5 to 30 nM for hepatitis B DNA, and from 0.3 to 1 mu M for dopamine. In our perception, this method represents a universal platform for the detection of analytes for which complementary oligomers or aptamers are available.
关键词Fluorescence Electrolytes Aggregation Ssdna Complementary Oligonucleotide Hepatitis b Virus Dopamine Dopamine-binding Aptamer
WOS标题词Science & Technology ; Physical Sciences
DOI10.1007/s00604-017-2486-7
关键词[WOS]GOLD NANOPARTICLES ; LABEL-FREE ; ELECTRODE ; POLYETHYLENEIMINE ; BIOSENSOR ; RECOVERY ; PLATFORM ; IONS ; ACID ; DOTS
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21405093) ; Scientific Research Foundation of Qufu Normal University(BSQD20130117)
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000412901100023
出版者SPRINGER WIEN
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/9826
专题中国科学院西北高原生物研究所
通讯作者Qu, Fei; You, Jinmao
作者单位1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式
GB/T 7714
Qu, Fei,Liu, Yining,Kong, Rongmei,et al. A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine[J]. MICROCHIMICA ACTA,2017,184(11):4417-4424.
APA Qu, Fei,Liu, Yining,Kong, Rongmei,&You, Jinmao.(2017).A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine.MICROCHIMICA ACTA,184(11),4417-4424.
MLA Qu, Fei,et al."A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine".MICROCHIMICA ACTA 184.11(2017):4417-4424.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qu, Fei]的文章
[Liu, Yining]的文章
[Kong, Rongmei]的文章
百度学术
百度学术中相似的文章
[Qu, Fei]的文章
[Liu, Yining]的文章
[Kong, Rongmei]的文章
必应学术
必应学术中相似的文章
[Qu, Fei]的文章
[Liu, Yining]的文章
[Kong, Rongmei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。