RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

doi:10.1038/s41598-017-13381-5

NWIPB OpenIR

	RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification
	Gao, Lei 1,2; Jia, Gongxue 3; Li, Ai 4; Ma, Haojia 1,2; Huang, Zhengyuan 1,2; Zhu, Shien 1,2; Hou, Yunpeng 5; Fu, Xiangwei 1,2
	2017-10-16
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7
文章类型	Article
摘要	In vitro maturation (IVM) and vitrification have been widely used to prepare oocytes before fertilization; however, potential effects of these procedures, such as expression profile changes, are poorly understood. In this study, mouse oocytes were divided into four groups and subjected to combinations of in vitro maturation and/or vitrification treatments. RNA-seq and in silico pathway analysis were used to identify differentially expressed genes (DEGs) that may be involved in oocyte viability after in vitro maturation and/or vitrification. Our results showed that 1) 69 genes were differentially expressed after IVM, 66 of which were up-regulated. Atp5e and Atp5o were enriched in the most significant gene ontology term "mitochondrial membrane part"; thus, these genes may be promising candidate biomarkers for oocyte viability after IVM. 2) The influence of vitrification on the transcriptome of oocytes was negligible, as no DEGs were found between vitrified and fresh oocytes. 3) The MII stage is more suitable for oocyte vitrification with respect to the transcriptome. This study provides a valuable new theoretical basis to further improve the efficiency of in vitro maturation and/or oocyte vitrification.
WOS标题词	Science & Technology
DOI	10.1038/s41598-017-13381-5
关键词[WOS]	MITOCHONDRIAL ATP SYNTHASE ; METAPHASE II OOCYTES ; BOVINE OOCYTES ; ASSISTED REPRODUCTION ; GERMINAL VESICLE ; DONOR OOCYTES ; FRESH ; GENE ; VIVO ; CRYOPRESERVATION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31101714 ; CAS ; 31372307)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000413048000056
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9827
专题	中国科学院西北高原生物研究所
通讯作者	Fu, Xiangwei
作者单位	1.China Agr Univ, Natl Engn Lab Anim Breeding, Minist Agr, Coll Anim Sci & Technol, Beijing 100193, Peoples R China 2.China Agr Univ, Key Lab Anim Genet Breeding & Reprod, Minist Agr, Coll Anim Sci & Technol, Beijing 100193, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 4.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Gao, Lei,Jia, Gongxue,Li, Ai,et al. RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification[J]. SCIENTIFIC REPORTS,2017,7.
APA	Gao, Lei.,Jia, Gongxue.,Li, Ai.,Ma, Haojia.,Huang, Zhengyuan.,...&Fu, Xiangwei.(2017).RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification.SCIENTIFIC REPORTS,7.
MLA	Gao, Lei,et al."RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification".SCIENTIFIC REPORTS 7(2017).