NWIPB OpenIR
Bright and sensitive ratiometric fluorescent probe enabling endogenous FA imaging and mechanistic exploration of indirect oxidative damage due to FA in various living systems
Dou, Kun1; Chen, Guang1,2,3; Yu, Fabiao2; Liu, Yuxia1; Chen, Lingxin2; Cao, Ziping1; Chen, Tao3; Li, Yulin3; You, Jinmao1,2
2017-11-01
发表期刊CHEMICAL SCIENCE
ISSN2041-6520
卷号8期号:11页码:7851-7861
文章类型Article
摘要As a notorious toxin, formaldehyde (FA) poses an immense threat to human health. Aberrantly elevated FA levels lead to serious pathologies, including organ damage, neurodegeneration, and cancer. Unfortunately, current techniques limit FA imaging to general comparative studies, instead of a mechanistic exploration of its biological role, and this is presumably due to the lack of robust molecular tools for reporting FA in living systems. More importantly, despite being reductive, FA, however, can induce oxidative damage to organisms, thus providing a challenge to the mechanistic study of FA using fluorescence imaging. Herein, we presented the design and multi-application of a bright sensitive ratiometric fluorescent probe 1-(4-(1H-phenanthro[9,10-d] imidazol-2-yl) phenyl) but-3-en-1-amine (PIPBA). With a p-extended phenylphenanthroimidazole fluorophore and an allylamine group, PIPBA exhibited high quantum yield (phi = 0.62) in blue fluorescent emission and selective reactivity toward FA. When sensing FA, PIPBA transformed to PIBE, which is a product capable of releasing bright green fluorescence (phi = 0.51) with its enhanced intramolecular charge transfer (ICT). Transformation of PIPBA to PIBE contributed to 80 nm of red shift in emission wavelength and a highly sensitive ratiometric response (92.2-fold), as well as a quite low detection limit (0.84 mu M). PIPBA was successfully applied to various living systems, realizing, for the first time, ratiometric quantification (in cells), in vivo imaging (zebrafish), and living tissue imaging (vivisectional mouse under anaesthetic) of endogenous FA that was spontaneously generated by biological systems. Furthermore, with the aid of PIPBA, we obtained visual evidence for the oxidative damage of FA in both HeLa cells and renal tissue of a living mouse. The results demonstrated that FA exerted indirect oxidative damage by interacting with free radicals, thus producing more oxidizing species, which eventually caused aggravated oxidative damage to the organism. The indirect oxidative damage due to FA could be alleviated by an exogenous or endogenous antioxidant. The excellent behaviors of PIPBA demonstrate that a chemical probe can detect endogenous FA in cells/tissue/vivo, promising to be an effective tool for further exploration of the biological mechanism of FA in living systems.
WOS标题词Science & Technology ; Physical Sciences
DOI10.1039/c7sc03719h
关键词[WOS]AZA-COPE REACTION ; SELECTIVE DETECTION ; MASS-SPECTROMETRY ; FREE-RADICALS ; FATTY-ACIDS ; VITAMIN-E ; FORMALDEHYDE ; CELLS ; DNA ; STRESS
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21475074 ; Shandong Province Key Laboratory of Detection Technology for Tumor Markers(KLDTTM2015-6 ; 21403123 ; KLDTTM2015-9) ; 21375075 ; 1301595 ; 21402106 ; 21475075 ; 21275089 ; 21405093 ; 21405094 ; 81303179 ; 21305076)
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000413532800066
出版者ROYAL SOC CHEMISTRY
引用统计
被引频次:81[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/9829
专题中国科学院西北高原生物研究所
通讯作者Chen, Guang; You, Jinmao
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
推荐引用方式
GB/T 7714
Dou, Kun,Chen, Guang,Yu, Fabiao,et al. Bright and sensitive ratiometric fluorescent probe enabling endogenous FA imaging and mechanistic exploration of indirect oxidative damage due to FA in various living systems[J]. CHEMICAL SCIENCE,2017,8(11):7851-7861.
APA Dou, Kun.,Chen, Guang.,Yu, Fabiao.,Liu, Yuxia.,Chen, Lingxin.,...&You, Jinmao.(2017).Bright and sensitive ratiometric fluorescent probe enabling endogenous FA imaging and mechanistic exploration of indirect oxidative damage due to FA in various living systems.CHEMICAL SCIENCE,8(11),7851-7861.
MLA Dou, Kun,et al."Bright and sensitive ratiometric fluorescent probe enabling endogenous FA imaging and mechanistic exploration of indirect oxidative damage due to FA in various living systems".CHEMICAL SCIENCE 8.11(2017):7851-7861.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Dou, Kun]的文章
[Chen, Guang]的文章
[Yu, Fabiao]的文章
百度学术
百度学术中相似的文章
[Dou, Kun]的文章
[Chen, Guang]的文章
[Yu, Fabiao]的文章
必应学术
必应学术中相似的文章
[Dou, Kun]的文章
[Chen, Guang]的文章
[Yu, Fabiao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。