Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug

doi:10.1016/j.bios.2017.08.009

NWIPB OpenIR

	Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug
	Kong, Weiheng 1,3; Wu, Di 4; Hu, Na2 ; Li, Ning 1,3; Dai, Chunji 1; Chen, Xuefeng 1; Suo, Yourui2 ; Li, Guoliang 1,3; Wu, Yongning 4
	2018-01-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	99 页码:653-659
文章类型	Article
摘要	Enzyme inhibition based drug screening strategy has been widely employed for new drug discovery. But this strategy faces some challenges in practical application especially for the trace active compound screening from natural products such as the stability of enzyme and the sensitivity of screening approach. Inspired by the above, we for the first time demonstrate the self-assembly of a-glucosidase (GAA) and glucose oxidase (GOx) into one multi-enzymes-inorganic nanoreactor with hierarchical structure (flower shape). The hybrid enzyme nanoreactor enjoys the merits including the character of assembly line, the enhanced enzymatic activity and robust stability. The flower shape of enzyme nanoreactor possessed a bigger specific surface area, facilitating the trace GAA inhibitor detection. Based on the above, we proposed an enzyme nanoreactor mediated plasmonic sensing strategy for anti-diabetic drug screening. First, maltose was chosen as the substrate for GAA and the generated glucose were immediately utilized by GOx to generate H2O2, and finally, H2O2 etched the Ag nanoprism to round nanodiscs, resulting in the blue shift of surface plasmon resonance (SPR) absorption band. With the aid of hybrid enzyme nanoreactor guided SPR, the ultrasensitive screening of GAA inhibitor (i.e. anti-diabetic drug) can be realized with the detection limit of 5 nM for acarbose. The proposed approach was successfully utilized for GAA inhibitor screening from natural products. We anticipate that the proposed sensing method may provide new insights and inspirations in the enzyme inhibition based drug discovery and clinical diagnosis.
关键词	Hybrid Enzyme Hierarchical Structure Alpha-glucosidase Plasmonic Sensing Anti-diabetic Drug
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.bios.2017.08.009
关键词[WOS]	IMMOBILIZATION ; NANOPARTICLES
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21677085 ; Natural Science Foundation of Shandong Province(ZR201702150005) ; China Postdoctoral Science Foundation(2016M590071) ; 21537001 ; 81472986)
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000413284200084
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9830
专题	中国科学院西北高原生物研究所
通讯作者	Li, Guoliang
作者单位	1.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 3.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 4.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Kong, Weiheng,Wu, Di,Hu, Na,et al. Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug[J]. BIOSENSORS & BIOELECTRONICS,2018,99:653-659.
APA	Kong, Weiheng.,Wu, Di.,Hu, Na.,Li, Ning.,Dai, Chunji.,...&Wu, Yongning.(2018).Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug.BIOSENSORS & BIOELECTRONICS,99,653-659.
MLA	Kong, Weiheng,et al."Robust hybrid enzyme nanoreactor mediated plasmonic sensing strategy for ultrasensitive screening of anti-diabetic drug".BIOSENSORS & BIOELECTRONICS 99(2018):653-659.