Selectively screen the antibacterial peptide from the hydrolysates of highland barley

doi:10.1002/elsc.201700118

NWIPB OpenIR

	Selectively screen the antibacterial peptide from the hydrolysates of highland barley
	Pei, Jinjin 1,2,3; Feng, Zhenzhen 1; Ren, Ting 1; Jin, Wengang 1; Li, Xinsheng 1; Chen, Dejing 1; Tao, Yanduo 2,3; Dang, Jun 2,3
	2018
发表期刊	ENGINEERING IN LIFE SCIENCES
ISSN	1618-0240
卷号	18 期号:1 页码:48-54
文章类型	Article
摘要	Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane.
关键词	Antibacterial Peptide Characterization Highland Barley Magnetic Liposome Purification
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/elsc.201700118
关键词[WOS]	ANTIMICROBIAL PEPTIDES ; STAPHYLOCOCCUS-AUREUS ; BACTERIOCIN ; THERMODYNAMICS ; CHROMATOGRAPHY ; CONSEQUENCES ; PURIFICATION ; BINDING ; NISIN
收录类别	SCI
语种	英语
项目资助者	Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Collaborative Innovation Center Research Foundation(QBXT-Z(P)-15-28) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Scientists' Foundations of Shaanxi University of Technology(SLGKYQD2014-2-18) ; Research Foundations of Shaanxi University of Technology(SLGQD13-13)
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000419053900005
出版者	WILEY
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/10382
专题	中国科学院西北高原生物研究所
通讯作者	Pei, Jinjin
作者单位	1.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhogn 723001, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 3.Qinghai Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018,18(1):48-54.
APA	Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES,18(1),48-54.
MLA	Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES 18.1(2018):48-54.