NWIPB OpenIR
Selectively screen the antibacterial peptide from the hydrolysates of highland barley
Pei, Jinjin1,2,3; Feng, Zhenzhen1; Ren, Ting1; Jin, Wengang1; Li, Xinsheng1; Chen, Dejing1; Tao, Yanduo2,3; Dang, Jun2,3
2018
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Volume18Issue:1Pages:48-54
SubtypeArticle
AbstractHighland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane.
KeywordAntibacterial Peptide Characterization Highland Barley Magnetic Liposome Purification
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/elsc.201700118
WOS KeywordANTIMICROBIAL PEPTIDES ; STAPHYLOCOCCUS-AUREUS ; BACTERIOCIN ; THERMODYNAMICS ; CHROMATOGRAPHY ; CONSEQUENCES ; PURIFICATION ; BINDING ; NISIN
Indexed BySCI
Language英语
Funding OrganizationResearch Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Collaborative Innovation Center Research Foundation(QBXT-Z(P)-15-28) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Scientists' Foundations of Shaanxi University of Technology(SLGKYQD2014-2-18) ; Research Foundations of Shaanxi University of Technology(SLGQD13-13)
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000419053900005
PublisherWILEY
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/10382
Collection中国科学院西北高原生物研究所
Corresponding AuthorPei, Jinjin
Affiliation1.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhogn 723001, Shaanxi, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
3.Qinghai Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018,18(1):48-54.
APA Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES,18(1),48-54.
MLA Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES 18.1(2018):48-54.
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