Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Selectively screen the antibacterial peptide from the hydrolysates of highland barley | |
Pei, Jinjin1,2,3; Feng, Zhenzhen1; Ren, Ting1; Jin, Wengang1; Li, Xinsheng1; Chen, Dejing1; Tao, Yanduo2,3; Dang, Jun2,3 | |
2018 | |
发表期刊 | ENGINEERING IN LIFE SCIENCES |
ISSN | 1618-0240 |
卷号 | 18期号:1页码:48-54 |
文章类型 | Article |
摘要 | Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane. |
关键词 | Antibacterial Peptide Characterization Highland Barley Magnetic Liposome Purification |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1002/elsc.201700118 |
关键词[WOS] | ANTIMICROBIAL PEPTIDES ; STAPHYLOCOCCUS-AUREUS ; BACTERIOCIN ; THERMODYNAMICS ; CHROMATOGRAPHY ; CONSEQUENCES ; PURIFICATION ; BINDING ; NISIN |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Collaborative Innovation Center Research Foundation(QBXT-Z(P)-15-28) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Scientists' Foundations of Shaanxi University of Technology(SLGKYQD2014-2-18) ; Research Foundations of Shaanxi University of Technology(SLGQD13-13) |
WOS研究方向 | Biotechnology & Applied Microbiology |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000419053900005 |
出版者 | WILEY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/10382 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Pei, Jinjin |
作者单位 | 1.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhogn 723001, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 3.Qinghai Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018,18(1):48-54. |
APA | Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES,18(1),48-54. |
MLA | Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES 18.1(2018):48-54. |
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