Knowledge Management System of Northwest Institute of Plateau Biology, CAS
2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry | |
Wang, Yuwei1,2,3; Luan, Guangxiang1,3; Zhou, Wu1,2,3; You, Jinmao1,2![]() ![]() ![]() | |
2018-02-01 | |
发表期刊 | FOOD ANALYTICAL METHODS
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ISSN | 1936-9751 |
卷号 | 11期号:2页码:451-465 |
文章类型 | Article |
摘要 | A novel method has been established for the rapid separation and determination of free fatty acids from 37 different varieties of raspberry. In this study, a new fluorescent labeling reagent for fatty acids, 2-(4-amino)-phenyl-1-hydrogen-phenanthrene [9, 10-d] imidazole (PIA), has been synthesized and successfully applied to the high-performance liquid chromatography (HPLC) determination of fatty acids in raspberry. The novel method has been optimized by HPLC with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). The 22 main fatty acids (FAs) present in raspberry were derivatized by PIA and separated on a reversed-phase Hypersil GOLD column with gradient elution. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). Under the optimum conditions, the method was successfully applied for the analysis of 22 fatty acids in 37 different varieties of raspberry. Good linear correlations were observed for all fatty acids with correlation coefficients of > 0.9978. Limits of detection and quantification (LOD and LOQ) were in the range of 0.12 to 0.49 ng/mL and 1.07 to 2.81 ng/mL, respectively. Furthermore, the results indicated that the raspberries were rich in fatty acids, but the contents of the fatty acids varied among the different varieties. |
关键词 | High-performance Liquid Chromatography With Fluorescence Detection And Online Mass Spectrometry Identification Pre-column Derivatization Raspberry Fatty Acids 2-(4-amino)-phenyl-1-hydrogen-phenanthrene [9 10-d] Imidazole |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s12161-017-1016-x |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; ANTIOXIDANT CAPACITY ; RED RASPBERRIES ; PHENOLICS ; OMEGA-3-FATTY-ACIDS ; DERIVATIZATION ; ELLAGITANNINS ; EXTRACTION ; FLAVONOIDS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | major science and technology projects of Qinghai province: utilization and development with high value and further process technology of medlar(2015-NK-A2) ; unit of science and technology office of Qinghai province: manufacture integration technology and demonstration of high standards medlar with antioxidant and anti-cohesion(2015-SF-120) ; Qinghai Provincial Science Foundation(2016-ZJ-928Q) ; CAS "Light of West China" Program: Study on the effect of Lycium ruthenicum Murr. anthocyanin on the liver - protecting |
WOS研究方向 | Food Science & Technology |
WOS类目 | Food Science & Technology |
WOS记录号 | WOS:000419178200015 |
出版者 | SPRINGER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/10383 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Hu, Na; Suo, Yourui |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yuwei,Luan, Guangxiang,Zhou, Wu,et al. 2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry[J]. FOOD ANALYTICAL METHODS,2018,11(2):451-465. |
APA | Wang, Yuwei,Luan, Guangxiang,Zhou, Wu,You, Jinmao,Hu, Na,&Suo, Yourui.(2018).2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry.FOOD ANALYTICAL METHODS,11(2),451-465. |
MLA | Wang, Yuwei,et al."2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry".FOOD ANALYTICAL METHODS 11.2(2018):451-465. |
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