2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry

doi:10.1007/s12161-017-1016-x

NWIPB OpenIR

	2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry
	Wang, Yuwei 1,2,3; Luan, Guangxiang 1,3; Zhou, Wu 1,2,3; You, Jinmao1,2 ; Hu, Na1,2 ; Suo, Yourui1,2
	2018-02-01
发表期刊	FOOD ANALYTICAL METHODS
ISSN	1936-9751
卷号	11 期号:2 页码:451-465
文章类型	Article
摘要	A novel method has been established for the rapid separation and determination of free fatty acids from 37 different varieties of raspberry. In this study, a new fluorescent labeling reagent for fatty acids, 2-(4-amino)-phenyl-1-hydrogen-phenanthrene [9, 10-d] imidazole (PIA), has been synthesized and successfully applied to the high-performance liquid chromatography (HPLC) determination of fatty acids in raspberry. The novel method has been optimized by HPLC with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS/MS). The 22 main fatty acids (FAs) present in raspberry were derivatized by PIA and separated on a reversed-phase Hypersil GOLD column with gradient elution. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). Under the optimum conditions, the method was successfully applied for the analysis of 22 fatty acids in 37 different varieties of raspberry. Good linear correlations were observed for all fatty acids with correlation coefficients of > 0.9978. Limits of detection and quantification (LOD and LOQ) were in the range of 0.12 to 0.49 ng/mL and 1.07 to 2.81 ng/mL, respectively. Furthermore, the results indicated that the raspberries were rich in fatty acids, but the contents of the fatty acids varied among the different varieties.
关键词	High-performance Liquid Chromatography With Fluorescence Detection And Online Mass Spectrometry Identification Pre-column Derivatization Raspberry Fatty Acids 2-(4-amino)-phenyl-1-hydrogen-phenanthrene [9 10-d] Imidazole
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s12161-017-1016-x
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; ANTIOXIDANT CAPACITY ; RED RASPBERRIES ; PHENOLICS ; OMEGA-3-FATTY-ACIDS ; DERIVATIZATION ; ELLAGITANNINS ; EXTRACTION ; FLAVONOIDS
收录类别	SCI
语种	英语
项目资助者	major science and technology projects of Qinghai province: utilization and development with high value and further process technology of medlar(2015-NK-A2) ; unit of science and technology office of Qinghai province: manufacture integration technology and demonstration of high standards medlar with antioxidant and anti-cohesion(2015-SF-120) ; Qinghai Provincial Science Foundation(2016-ZJ-928Q) ; CAS "Light of West China" Program: Study on the effect of Lycium ruthenicum Murr. anthocyanin on the liver - protecting
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000419178200015
出版者	SPRINGER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/10383
专题	中国科学院西北高原生物研究所
通讯作者	Hu, Na; Suo, Yourui
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yuwei,Luan, Guangxiang,Zhou, Wu,et al. 2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry[J]. FOOD ANALYTICAL METHODS,2018,11(2):451-465.
APA	Wang, Yuwei,Luan, Guangxiang,Zhou, Wu,You, Jinmao,Hu, Na,&Suo, Yourui.(2018).2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry.FOOD ANALYTICAL METHODS,11(2),451-465.
MLA	Wang, Yuwei,et al."2-(4-Amino)-Phenyl-1-Hydrogen-Phenanthrene [9,10-d] Imidazole as a Novel Fluorescent Labeling Reagent for Determination of Fatty Acids in Raspberry".FOOD ANALYTICAL METHODS 11.2(2018):451-465.