Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae) | |
| Zhang, Qian1; Yang, Yan-Zhuo1; Wu, Gui-Li1; Zhang, Dong-Yuan1; Liu, Jian-Quan1,2 | |
| 2008-06-01 | |
| 发表期刊 | CONSERVATION GENETICS
 |
| 卷号 | 9期号:3页码:767-769 |
| 文章类型 | Article |
| 摘要 | Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.; Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species. |
| 关键词 | Juniperus Przewalskii Microsatellite Markers Genetic Diversity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科领域 | 遗传学 |
| 关键词[WOS] | LOCI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biodiversity & Conservation ; Genetics & Heredity |
| WOS类目 | Biodiversity Conservation ; Genetics & Heredity |
| WOS记录号 | WOS:000255191000035 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/1221 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, MOE Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,et al. Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)[J]. CONSERVATION GENETICS,2008,9(3):767-769. |
| APA | Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,Zhang, Dong-Yuan,&Liu, Jian-Quan.(2008).Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae).CONSERVATION GENETICS,9(3),767-769. |
| MLA | Zhang, Qian,et al."Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)".CONSERVATION GENETICS 9.3(2008):767-769. |
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