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Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)
Zhang, Qian1; Yang, Yan-Zhuo1; Wu, Gui-Li1; Zhang, Dong-Yuan1; Liu, Jian-Quan1,2
2008-06-01
Source PublicationCONSERVATION GENETICS
Volume9Issue:3Pages:767-769
SubtypeArticle
AbstractJuniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.; Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.
KeywordJuniperus Przewalskii Microsatellite Markers Genetic Diversity
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Subject Area遗传学
WOS KeywordLOCI
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000255191000035
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1221
Collection中国科学院西北高原生物研究所
Affiliation1.Lanzhou Univ, Sch Life Sci, MOE Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,et al. Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)[J]. CONSERVATION GENETICS,2008,9(3):767-769.
APA Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,Zhang, Dong-Yuan,&Liu, Jian-Quan.(2008).Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae).CONSERVATION GENETICS,9(3),767-769.
MLA Zhang, Qian,et al."Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)".CONSERVATION GENETICS 9.3(2008):767-769.
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