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LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology | |
Zhao, Xianen1,2; Suo, Yourui1 | |
2008-03-01 | |
发表期刊 | CHROMATOGRAPHIA
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卷号 | 67期号:5-6页码:375-382 |
文章类型 | Article |
摘要 | An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.; An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples. |
关键词 | Column Liquid Chromatography-mass Spectrometry AmIno Acids In Rat Plasma 1 2-benzo-3 4-dihydrocarbazole-9-ethyl Chloroformate |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
学科领域 | 生物科学 |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; PRECOLUMN DERIVATIZATION ; HEPATITIS ; FATIGUE ; REAGENT |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000253624100005 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1231 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Xianen,Suo, Yourui. LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology[J]. CHROMATOGRAPHIA,2008,67(5-6):375-382. |
APA | Zhao, Xianen,&Suo, Yourui.(2008).LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA,67(5-6),375-382. |
MLA | Zhao, Xianen,et al."LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology".CHROMATOGRAPHIA 67.5-6(2008):375-382. |
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