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LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology
Zhao, Xianen1,2; Suo, Yourui1
2008-03-01
发表期刊CHROMATOGRAPHIA
卷号67期号:5-6页码:375-382
文章类型Article
摘要An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.; An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.
关键词Column Liquid Chromatography-mass Spectrometry AmIno Acids In Rat Plasma 1 2-benzo-3 4-dihydrocarbazole-9-ethyl Chloroformate
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域生物科学
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; PRECOLUMN DERIVATIZATION ; HEPATITIS ; FATIGUE ; REAGENT
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000253624100005
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1231
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Xianen,Suo, Yourui. LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology[J]. CHROMATOGRAPHIA,2008,67(5-6):375-382.
APA Zhao, Xianen,&Suo, Yourui.(2008).LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA,67(5-6),375-382.
MLA Zhao, Xianen,et al."LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology".CHROMATOGRAPHIA 67.5-6(2008):375-382.
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