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LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology
Zhao, Xianen1,2; Suo, Yourui1
2008-03-01
Source PublicationCHROMATOGRAPHIA
Volume67Issue:5-6Pages:375-382
SubtypeArticle
AbstractAn LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.; An LC method for the determination of 20 amino acids (AAs), using 1,2-Benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as fluorescent labeling reagent, has been validated and applied for the analysis of AAs in rat plasma at three different states concerning exercise physiology. Identification of AA derivatives was carried out by LC-MS with electrospray ion (ESI), and the MS-MS cleavage mode of the representative tyrosine (Tyr) derivative was analyzed. Gradient elution on a Hypersil BDS C-18 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 50-200 mu L of plasma samples. The contents of 20 AAs in rat plasma of three groups (24 rats, group A: quiet state, group B: at exercising exhaust, group C: 12 h after exercising exhaust) exhibited evident difference corresponding to the physiological states. Facile BCEOC derivatization coupled with LC-FLD-ESI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of AAs from plasma or other biochemical samples.
KeywordColumn Liquid Chromatography-mass Spectrometry AmIno Acids In Rat Plasma 1 2-benzo-3 4-dihydrocarbazole-9-ethyl Chloroformate
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Subject Area生物科学
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; PRECOLUMN DERIVATIZATION ; HEPATITIS ; FATIGUE ; REAGENT
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000253624100005
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1231
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Xianen,Suo, Yourui. LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology[J]. CHROMATOGRAPHIA,2008,67(5-6):375-382.
APA Zhao, Xianen,&Suo, Yourui.(2008).LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology.CHROMATOGRAPHIA,67(5-6),375-382.
MLA Zhao, Xianen,et al."LC determination of amino acids in rat plasma with fluorescence detection: Application to exercise physiology".CHROMATOGRAPHIA 67.5-6(2008):375-382.
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