Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS | |
Wang, Li; Xiao, Hongbin; Liang, Xinmiao; Wei, Lixin | |
2007-07-01 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE |
卷号 | 30期号:10页码:1488-1495 |
文章类型 | Article |
摘要 | An HPLC-UV-MS method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in Gastrodia elata was developed, which was based on their UV and MS characteristics summarized through a series of homemade reference standard experiments. Phenolics showed characteristic UV lambda(max) at 267 nm, [M + NH4](+) base peak in positive mode and [M - H](-) base peak in negative mode while nucleosides exhibited UV lambda(max) at 255 nm, [M + H](+), [M - H + 2H(2)O](-) or [M - H + CH3COOH](-). Phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 U) and the combined loss of H2O and CO2 from the citric acid unit under negative MS/MS conditions whereas nucleosides simply lost the ribose (- 132 U) under positive MS/MS conditions. According to these characteristics, a special pattern under MS/MS conditions and reported compound data for G. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. Among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from G. elata.; An HPLC-UV-MS method for simultaneous identification of predominant phenolics and minor nucleoside derivatives in Gastrodia elata was developed, which was based on their UV and MS characteristics summarized through a series of homemade reference standard experiments. Phenolics showed characteristic UV lambda(max) at 267 nm, [M + NH4](+) base peak in positive mode and [M - H](-) base peak in negative mode while nucleosides exhibited UV lambda(max) at 255 nm, [M + H](+), [M - H + 2H(2)O](-) or [M - H + CH3COOH](-). Phenolics conjugates mainly underwent the consecutive loss of gastrodin residue (- 268 U) and the combined loss of H2O and CO2 from the citric acid unit under negative MS/MS conditions whereas nucleosides simply lost the ribose (- 132 U) under positive MS/MS conditions. According to these characteristics, a special pattern under MS/MS conditions and reported compound data for G. elata in the literature, not only 15 phenolics were identified but also 6 nucleoside derivatives were identified. Among these compounds, seven phenolics and three nucleoside derivatives have not been reported yet from G. elata. |
关键词 | Gastrodia Elata Lc-ms Nucleoside Phenolic |
WOS标题词 | Science & Technology ; Physical Sciences |
学科领域 | 生物科学 |
关键词[WOS] | P-HYDROXYBENZYL ALCOHOL ; CAPILLARY-ELECTROPHORESIS ; MASS-SPECTROMETRY ; PARISHIN-B ; BLUME ; CHROMATOGRAPHY ; INHIBITION ; RHIZOMA |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000248240000013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1262 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Res Ctr Tibetan Med, NW Plateau Inst Biol, Xining, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Li,Xiao, Hongbin,Liang, Xinmiao,et al. Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1488-1495. |
APA | Wang, Li,Xiao, Hongbin,Liang, Xinmiao,&Wei, Lixin.(2007).Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS.JOURNAL OF SEPARATION SCIENCE,30(10),1488-1495. |
MLA | Wang, Li,et al."Identification of phenolics and nucleoside derivatives in Gastrodia elata by HPLC-UV-MS".JOURNAL OF SEPARATION SCIENCE 30.10(2007):1488-1495. |
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