NWIPB OpenIR
Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines
You, Jinmao; Ding, Chenxu; Zhu, Fang; Sun, Xuejun; Li, Yulin; Suo, Yourui
2007-05-01
发表期刊CHROMATOGRAPHIA
卷号65期号:9-10页码:545-553
文章类型Article
摘要A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone.; A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone.
关键词Column Liquid Chromatography-mass Spectrometry Tibetan Herbal Medicines Phenolic Compounds Dansyl Derivatization
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
学科领域生物化学
关键词[WOS]FLAVONOIDS ; FRAGMENTATION ; RESOLUTION ; EXTRACTS ; UV ; MS
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000246878900007
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1276
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714
You, Jinmao,Ding, Chenxu,Zhu, Fang,et al. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines[J]. CHROMATOGRAPHIA,2007,65(9-10):545-553.
APA You, Jinmao,Ding, Chenxu,Zhu, Fang,Sun, Xuejun,Li, Yulin,&Suo, Yourui.(2007).Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines.CHROMATOGRAPHIA,65(9-10),545-553.
MLA You, Jinmao,et al."Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines".CHROMATOGRAPHIA 65.9-10(2007):545-553.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2007Mass Spectrometr(446KB) 开放获取使用许可浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[You, Jinmao]的文章
[Ding, Chenxu]的文章
[Zhu, Fang]的文章
百度学术
百度学术中相似的文章
[You, Jinmao]的文章
[Ding, Chenxu]的文章
[Zhu, Fang]的文章
必应学术
必应学术中相似的文章
[You, Jinmao]的文章
[Ding, Chenxu]的文章
[Zhu, Fang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 2007Mass Spectrometric Identification.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。