Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines | |
You, Jinmao; Ding, Chenxu; Zhu, Fang; Sun, Xuejun; Li, Yulin; Suo, Yourui | |
2007-05-01 | |
发表期刊 | CHROMATOGRAPHIA |
卷号 | 65期号:9-10页码:545-553 |
文章类型 | Article |
摘要 | A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone.; A highly selective and accurate method based on derivatization with dansyl chloride coupled with liquid chromatography-mass spectrometry has been developed for identification of natural pharmacologically active phenolic compounds in extracts of Lomatogonium rotatum plants (Tibetan herbal medicine) obtained by solid-phase extraction. The number of hydroxyl groups on the dansylated phenols was estimated by LC-MS-MS analysis in positive-ion mode. Dansyl derivatization of the compounds introduced basic secondary nitrogen into the phenolic core structures and this was readily ionized when acidic HPLC mobile phases were used. MS fragmentation of the derivatives generated intense protonated molecular ions of m/z [MH](+) (phenol aglycones were transformed into the corresponding free phenols by cleavage of an aglycone bond). Collision-induced dissociation of the protonated molecule generated characteristic product ions of m/z 234 and 171 corresponding to the protonated 5-(dimethylamino)naphthalene sulfoxide and 5 -(dimethylamino) naphthalene moieties, respectively. Selected reaction monitoring based on the m/z [MH](+) to 234 and 171 transitions was highly specific for these phenolic compounds. Characteristic ions with m/z values of [MH - 234](+), [MH 2 x 234](+), and [MH - 3 x 234](+) were of great importance for estimation of the presence of multihydroxyl groups on the phenolic backbone. |
关键词 | Column Liquid Chromatography-mass Spectrometry Tibetan Herbal Medicines Phenolic Compounds Dansyl Derivatization |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
学科领域 | 生物化学 |
关键词[WOS] | FLAVONOIDS ; FRAGMENTATION ; RESOLUTION ; EXTRACTS ; UV ; MS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000246878900007 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1276 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China |
推荐引用方式 GB/T 7714 | You, Jinmao,Ding, Chenxu,Zhu, Fang,et al. Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines[J]. CHROMATOGRAPHIA,2007,65(9-10):545-553. |
APA | You, Jinmao,Ding, Chenxu,Zhu, Fang,Sun, Xuejun,Li, Yulin,&Suo, Yourui.(2007).Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines.CHROMATOGRAPHIA,65(9-10),545-553. |
MLA | You, Jinmao,et al."Mass spectrometric identification of multihydroxy phenolic compounds in Tibetan herbal medicines".CHROMATOGRAPHIA 65.9-10(2007):545-553. |
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