Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization | |
Ma, Tao1,2,3; Hu, Na1,2; Zhang, Qiulong1,2,3; Ding, Chenxi4; Che, Guodong1,2; Wu, Hongliang1,2; Sun, Yanan1,2,3; Ji, Zhongyin1,2,3; You, Jinmao1,2; Suo, Yourui1,2; Ding, Chenxu1,2,4 | |
2018 | |
发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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卷号 | 41期号:2页码:49-57 |
文章类型 | Article |
摘要 | A rapid and sensitive reversed-phase high performance liquid chromatography- fluorescence-mass spectrometer (RP-HPLC-FLD-MS) method based on pre-column derivatization using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as labelling reagent has been developed for simultaneous determination of five triterpenic acids (asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA), and betulinic acid (BA). The presented method was validated for linearity (correlation coefficient R-2>0.9994), precision (RSD 2.1%-3.9%) and reproducibility (RSD 0.01%-2.1%). The limits of detection (LODs) and the limits of quantitation LOQs were within the range of 0.71-1.02 ng mL(-1) and 2.28-3.24 ng mL(-1), respectively. The proposed method was successfully applied to simultaneously determine five triterpenic acids of four Corydalis plants and showed satisfactory reproducibility and credibility. Moreover, several main parameters affecting extraction procedure and derivatization efficiency were investigated by response surface methodology (RSM), respectively. Triterpenic acid content in four Corydalis plants was measured according to the established method and the results indicated that triterpenic acid contents were various in different organs and herbs. |
关键词 | Corydalis Microwave Assisted Extraction Pre-column Derivatization Response Surface Methodology Rp-hplc-fld-ms Triterpenic Acids |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1080/10826076.2015.1116092 |
关键词[WOS] | ISOQUINOLINE ALKALOIDS ; URSOLIC ACID ; CHEMOPREVENTIVE ACTIVITY ; QUANTIFICATION ; DERIVATIVES |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Key Project of Chinese Ministry of Education(212188) ; Innovation platform for the development and construction of special project of Key Laboratory of Tibetan Medicine Research of Qinghai Province(2017-ZJ-Y11) ; Qinghai Provincial Science Foundation(2014-NS-516 ; 2015-SF-123) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000424808500001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/13333 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810001, Qinghai, Peoples R China 2.Qinghai Prov Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qinghai Red Cross Hosp, Xining, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Tao,Hu, Na,Zhang, Qiulong,et al. Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018,41(2):49-57. |
APA | Ma, Tao.,Hu, Na.,Zhang, Qiulong.,Ding, Chenxi.,Che, Guodong.,...&Ding, Chenxu.(2018).Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,41(2),49-57. |
MLA | Ma, Tao,et al."Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 41.2(2018):49-57. |
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