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Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization
Ma, Tao1,2,3; Hu, Na1,2; Zhang, Qiulong1,2,3; Ding, Chenxi4; Che, Guodong1,2; Wu, Hongliang1,2; Sun, Yanan1,2,3; Ji, Zhongyin1,2,3; You, Jinmao1,2; Suo, Yourui1,2; Ding, Chenxu1,2,4
2018
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume41Issue:2Pages:49-57
SubtypeArticle
AbstractA rapid and sensitive reversed-phase high performance liquid chromatography- fluorescence-mass spectrometer (RP-HPLC-FLD-MS) method based on pre-column derivatization using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as labelling reagent has been developed for simultaneous determination of five triterpenic acids (asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA), and betulinic acid (BA). The presented method was validated for linearity (correlation coefficient R-2>0.9994), precision (RSD 2.1%-3.9%) and reproducibility (RSD 0.01%-2.1%). The limits of detection (LODs) and the limits of quantitation LOQs were within the range of 0.71-1.02 ng mL(-1) and 2.28-3.24 ng mL(-1), respectively. The proposed method was successfully applied to simultaneously determine five triterpenic acids of four Corydalis plants and showed satisfactory reproducibility and credibility. Moreover, several main parameters affecting extraction procedure and derivatization efficiency were investigated by response surface methodology (RSM), respectively. Triterpenic acid content in four Corydalis plants was measured according to the established method and the results indicated that triterpenic acid contents were various in different organs and herbs.
KeywordCorydalis Microwave Assisted Extraction Pre-column Derivatization Response Surface Methodology Rp-hplc-fld-ms Triterpenic Acids
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1080/10826076.2015.1116092
WOS KeywordISOQUINOLINE ALKALOIDS ; URSOLIC ACID ; CHEMOPREVENTIVE ACTIVITY ; QUANTIFICATION ; DERIVATIVES
Indexed BySCI
Language英语
Funding OrganizationKey Project of Chinese Ministry of Education(212188) ; Innovation platform for the development and construction of special project of Key Laboratory of Tibetan Medicine Research of Qinghai Province(2017-ZJ-Y11) ; Qinghai Provincial Science Foundation(2014-NS-516 ; 2015-SF-123)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000424808500001
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/13333
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810001, Qinghai, Peoples R China
2.Qinghai Prov Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qinghai Red Cross Hosp, Xining, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Ma, Tao,Hu, Na,Zhang, Qiulong,et al. Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018,41(2):49-57.
APA Ma, Tao.,Hu, Na.,Zhang, Qiulong.,Ding, Chenxi.,Che, Guodong.,...&Ding, Chenxu.(2018).Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,41(2),49-57.
MLA Ma, Tao,et al."Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 41.2(2018):49-57.
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