Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC

doi:10.1007/s13205-018-1203-5

NWIPB OpenIR

	Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC
	Lei, Tianxiang 1,4; Wang, Huan 2; Li, Songling 5; Shen, Jianwei 2; Chen, Shilong 1,3; Cai, Xiaojian 5; Zhou, Dangwei 1,3,4
	2018-03-13
发表期刊	3 BIOTECH
卷号	8
文章类型	Article
摘要	To achieve a high yield of tropane alkaloids (TA) and exploit the alpine plant sustainably, an optimized protocol for induction and establishment of hairy roots culture of Prezwalskia tangutica Maxim was developed through selection of appropriate Agrobacterium strain and the explant type. The hypocotyl is more readily facile to induce the HR than the cotyledon is when infected with the three different agrobacterium strains. MUS440 has an efficiency (of up to 20%), whereas the ATCC10060 (A4) can induce HR on both types of explants with the highest frequency (33.33%), root length (21.17 +/- 2.84 cm), and root number (10.83 +/- 1.43) per explant than the other strains. The highest HR production resulted from using hypocotyl as explants. Independent transformed HR was able to grow vigorously and to propagate on a no-hormone 1/2MS liquid medium. The presence of pRi rolB gene in transformation of HR was confirmed by PCR amplification. In the liquid medium, the HR growth curve appeared to be "S" shaped, and ADB had increased to 4.633 g/l. Moreover, HPLC analysis showed that HR lines have an extraordinary ability to produce atropine (229.88 mg/100 g), anisodine (4.09 mg/100 g), anisodamine (12.85 mg/100 g), and scopolamine (10.69 mg/100 g), which were all more significant than the control roots. In conclusion, our study optimized the culture condition and established a feasible genetics reactor for P. tangutica green exploration and biological study in the alpine region.
关键词	Przewalskia Tangutica Maxim Hairy Root Tropane Alkaloid Hplc
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s13205-018-1203-5
关键词[WOS]	RHIZOGENES-MEDIATED TRANSFORMATION ; HAIRY-ROOT CULTURES ; AGROBACTERIUM-RHIZOGENES ; TROPANE ALKALOIDS ; ESTABLISHMENT
收录类别	SCI
语种	英语
项目资助者	National Foundation of Science(31070208) ; Training Qualified People Plan "Hope of West China''(Y229151211) ; Basic Research Programs of Science and Technology in Qinghai Province(2017-ZJ-702) ; Construction Project for Innovation Platform of Qinghai Province(2017-ZJ-Y14)
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000427628700001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13338
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Tibetan Med Ctr, Xining 810008, Qinghai, Peoples R China 3.Chinese Acad Sci, Key Lab Qinghai Plant Mol Breeding, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 5.Qinghai Univ, Inst Soil & Fertilizer, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Lei, Tianxiang,Wang, Huan,Li, Songling,et al. Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC[J]. 3 BIOTECH,2018,8.
APA	Lei, Tianxiang.,Wang, Huan.,Li, Songling.,Shen, Jianwei.,Chen, Shilong.,...&Zhou, Dangwei.(2018).Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC.3 BIOTECH,8.
MLA	Lei, Tianxiang,et al."Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC".3 BIOTECH 8(2018).