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Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system | |
Yan, Xiaolu1; Zhao, Xian-En1; Sun, Jing2; Zhu, Shuyun1,2; Lei, Cuihua1; Li, Ru1; Gong, Peiwei1; Ling, Baoping1; Wang, Renjun3; Wang, Hua1 | |
2018-06-15 | |
发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL |
卷号 | 263页码:27-35 |
文章类型 | Article |
摘要 | Graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) were combined for probing glutathione reductase (GR) activity. The main probing mechanism is based on the following facts: (1) The fluorescence of GQDs can be quenched by AuNPs through fluorescence resonance energy transfer (FRET). (2) Reduced glutathione (GSH) can protect AuNPs from being aggregated and enlarge the inter-particle distance between GQDs and AuNPs, which subsequently produces the fluorescent signal recovery. (3) AuNPs has much weaker affinity towards oxidized glutathione (GSSG) in contrast to GSH. (4) GR can catalyze the reduction of GSSG into GSH in the presence of coenzyme beta-nicotinamide adenine dinucleotide 2'-phophate hydrate (NADPH). Based on these facts, a turn-on fluorescent assay for GR has thus been developed based on the GQDs-AuNPs system. The proposed assay can allow for the evaluation of GR activity in the range of 0.0050-0.13 mU/mL, with a minimum detectable concentration of 0.0050 mU/mL, which was more than one order of magnitude lower than most of the current sensing platforms. Besides, this GQDs-AuNPs based GR assay was successfully applied for screening the inhibitor of GR activity by using 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) as a model. (C) 2018 Elsevier B.V. All rights reserved. |
关键词 | Graphene Quantum Dots Gold Nanoparticles Enzyme Catalysis Glutathione Reductase Inhibitor Screening |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
DOI | 10.1016/j.snb.2018.02.096 |
关键词[WOS] | QUANTITATIVE-DETERMINATION ; FLUORESCENT NANOSENSOR ; FLUOROMETRIC ASSAY ; LABEL-FREE ; OXIDE ; BIOTHIOLS ; ACID ; NANOCLUSTERS ; PLATFORM ; HYBRIDS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2017MC037 ; Project of Shandong Province Higher Educational Science and Technology Program(J16LA03) ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Development Project of Qinghai Key Laboratory(2017-ZJ-Y10) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Taishan Scholar Foundation of Shandong Province, China ; 21775088 ; ZR2016EMB04) ; 21675099 ; 21603116) |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
WOS记录号 | WOS:000427704400004 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/13342 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Inst Med & Mat Appl Technol, Qufu City 273165, Shandong, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 3.Qufu Normal Univ, Coll Life Sci, Qufu City 273165, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Yan, Xiaolu,Zhao, Xian-En,Sun, Jing,et al. Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,263:27-35. |
APA | Yan, Xiaolu.,Zhao, Xian-En.,Sun, Jing.,Zhu, Shuyun.,Lei, Cuihua.,...&Wang, Hua.(2018).Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system.SENSORS AND ACTUATORS B-CHEMICAL,263,27-35. |
MLA | Yan, Xiaolu,et al."Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system".SENSORS AND ACTUATORS B-CHEMICAL 263(2018):27-35. |
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