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Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system
Yan, Xiaolu1; Zhao, Xian-En1; Sun, Jing2; Zhu, Shuyun1,2; Lei, Cuihua1; Li, Ru1; Gong, Peiwei1; Ling, Baoping1; Wang, Renjun3; Wang, Hua1
2018-06-15
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号263页码:27-35
文章类型Article
摘要Graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) were combined for probing glutathione reductase (GR) activity. The main probing mechanism is based on the following facts: (1) The fluorescence of GQDs can be quenched by AuNPs through fluorescence resonance energy transfer (FRET). (2) Reduced glutathione (GSH) can protect AuNPs from being aggregated and enlarge the inter-particle distance between GQDs and AuNPs, which subsequently produces the fluorescent signal recovery. (3) AuNPs has much weaker affinity towards oxidized glutathione (GSSG) in contrast to GSH. (4) GR can catalyze the reduction of GSSG into GSH in the presence of coenzyme beta-nicotinamide adenine dinucleotide 2'-phophate hydrate (NADPH). Based on these facts, a turn-on fluorescent assay for GR has thus been developed based on the GQDs-AuNPs system. The proposed assay can allow for the evaluation of GR activity in the range of 0.0050-0.13 mU/mL, with a minimum detectable concentration of 0.0050 mU/mL, which was more than one order of magnitude lower than most of the current sensing platforms. Besides, this GQDs-AuNPs based GR assay was successfully applied for screening the inhibitor of GR activity by using 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) as a model. (C) 2018 Elsevier B.V. All rights reserved.
关键词Graphene Quantum Dots Gold Nanoparticles Enzyme Catalysis Glutathione Reductase Inhibitor Screening
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1016/j.snb.2018.02.096
关键词[WOS]QUANTITATIVE-DETERMINATION ; FLUORESCENT NANOSENSOR ; FLUOROMETRIC ASSAY ; LABEL-FREE ; OXIDE ; BIOTHIOLS ; ACID ; NANOCLUSTERS ; PLATFORM ; HYBRIDS
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2017MC037 ; Project of Shandong Province Higher Educational Science and Technology Program(J16LA03) ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Development Project of Qinghai Key Laboratory(2017-ZJ-Y10) ; State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Taishan Scholar Foundation of Shandong Province, China ; 21775088 ; ZR2016EMB04) ; 21675099 ; 21603116)
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000427704400004
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/13342
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Inst Med & Mat Appl Technol, Qufu City 273165, Shandong, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
3.Qufu Normal Univ, Coll Life Sci, Qufu City 273165, Shandong, Peoples R China
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GB/T 7714
Yan, Xiaolu,Zhao, Xian-En,Sun, Jing,et al. Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,263:27-35.
APA Yan, Xiaolu.,Zhao, Xian-En.,Sun, Jing.,Zhu, Shuyun.,Lei, Cuihua.,...&Wang, Hua.(2018).Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system.SENSORS AND ACTUATORS B-CHEMICAL,263,27-35.
MLA Yan, Xiaolu,et al."Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system".SENSORS AND ACTUATORS B-CHEMICAL 263(2018):27-35.
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