miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis

doi:10.7150/ijbs.25052

NWIPB OpenIR

	miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis
	Wang, Jian 1; Tan, Jiaoyan 1; Qi, Qi 1; Yang, Lingzhi 1; Wang, Yanhong 1; Zhang, Chunlei 1; Hu, Linyong2 ; Chen, Hong 1; Fang, Xingtang 1
	2018
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN	1449-2288
卷号	14 期号:7 页码:760-774
文章类型	Article
摘要	MicroRNAs are endogenous, small non-coding RNAs that can play critical gene-regulatory roles during skeletal muscle development and are highly conserved. miR-487b-3p is expressed in muscle, and the detailed mechanism by which it regulates myoblast proliferation and differentiation has not been explored. Here, we found that miR-487b-3p expression was significantly higher in goat muscle tissues than in other tissues and was higher in fetal goat muscle tissues than in mature goat tissues, suggesting that miR-487b-3p has an important effect on skeletal muscle myogenesis. Functional studies showed that miR-487b-3p overexpression significantly suppressed C2C12 myoblast proliferation and differentiation, which was accompanied by the down-regulation of functional genes related to proliferation (MyoD, Pax7 and PCNA) and differentiation (Myf5, MyoG and Mef2c), whereas the inhibition of miR-487b-3p accelerated C2C12 myoblast proliferation and differentiation and was accompanied by the up-regulation of functional genes. Using Target-Scan and David, we found that miR-487b-3p targeted the 3'-UTR of IRS1, an essential regulator in the PI3K/Akt and MAPK/Erk pathways. We then confirmed the targeting of IRS1 by miR-487b-3p using dual-luciferase assays, RT-qPCR and western blotting. Furthermore, IRS1 silencing markedly inhibited proliferation and differentiation in cultured C2C12 myoblasts, confirming the important role of IRS1 in myogenesis. These results reveal an IRS1 -mediated regulatory link between miR-487b-3p and the PI3K/Akt and MAPK/Erk pathways during skeletal muscle myogenesis.
关键词	Mir-487b-3p Irs1 Myoblast Proliferation Myoblast Differentiation Myogenesis
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.7150/ijbs.25052
关键词[WOS]	SATELLITE CELLS ; TRANSCRIPTION FACTORS ; PI3K/AKT PATHWAYS ; GROWTH-FACTOR ; EXPRESSION ; MICRORNAS ; MAPK/ERK ; CANCER ; PHOSPHORYLATION ; INHIBITION
收录类别	SCI
语种	英语
项目资助者	Science and Technology Planning Project of Jiangsu Province(SZ-XZ2017016 ; Science and Technology Planning Project of Xuzhou City(KC16NX073 ; Natural Science Foundation of Jiangsu Province(BK20160217) ; Applied Basic Research Program of Qinghai Province(2014-ZJ-710) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; BN2015027) ; KC15N0013)
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS类目	Biochemistry & Molecular Biology ; Biology
WOS记录号	WOS:000433261800007
出版者	IVYSPRING INT PUBL
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13823
专题	中国科学院西北高原生物研究所
通讯作者	Fang, Xingtang
作者单位	1.Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jian,Tan, Jiaoyan,Qi, Qi,et al. miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(7):760-774.
APA	Wang, Jian.,Tan, Jiaoyan.,Qi, Qi.,Yang, Lingzhi.,Wang, Yanhong.,...&Fang, Xingtang.(2018).miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(7),760-774.
MLA	Wang, Jian,et al."miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.7(2018):760-774.