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miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis
Wang, Jian1; Tan, Jiaoyan1; Qi, Qi1; Yang, Lingzhi1; Wang, Yanhong1; Zhang, Chunlei1; Hu, Linyong2; Chen, Hong1; Fang, Xingtang1
2018
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
卷号14期号:7页码:760-774
文章类型Article
摘要MicroRNAs are endogenous, small non-coding RNAs that can play critical gene-regulatory roles during skeletal muscle development and are highly conserved. miR-487b-3p is expressed in muscle, and the detailed mechanism by which it regulates myoblast proliferation and differentiation has not been explored. Here, we found that miR-487b-3p expression was significantly higher in goat muscle tissues than in other tissues and was higher in fetal goat muscle tissues than in mature goat tissues, suggesting that miR-487b-3p has an important effect on skeletal muscle myogenesis. Functional studies showed that miR-487b-3p overexpression significantly suppressed C2C12 myoblast proliferation and differentiation, which was accompanied by the down-regulation of functional genes related to proliferation (MyoD, Pax7 and PCNA) and differentiation (Myf5, MyoG and Mef2c), whereas the inhibition of miR-487b-3p accelerated C2C12 myoblast proliferation and differentiation and was accompanied by the up-regulation of functional genes. Using Target-Scan and David, we found that miR-487b-3p targeted the 3'-UTR of IRS1, an essential regulator in the PI3K/Akt and MAPK/Erk pathways. We then confirmed the targeting of IRS1 by miR-487b-3p using dual-luciferase assays, RT-qPCR and western blotting. Furthermore, IRS1 silencing markedly inhibited proliferation and differentiation in cultured C2C12 myoblasts, confirming the important role of IRS1 in myogenesis. These results reveal an IRS1 -mediated regulatory link between miR-487b-3p and the PI3K/Akt and MAPK/Erk pathways during skeletal muscle myogenesis.
关键词Mir-487b-3p Irs1 Myoblast Proliferation Myoblast Differentiation Myogenesis
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.7150/ijbs.25052
关键词[WOS]SATELLITE CELLS ; TRANSCRIPTION FACTORS ; PI3K/AKT PATHWAYS ; GROWTH-FACTOR ; EXPRESSION ; MICRORNAS ; MAPK/ERK ; CANCER ; PHOSPHORYLATION ; INHIBITION
收录类别SCI
语种英语
项目资助者Science and Technology Planning Project of Jiangsu Province(SZ-XZ2017016 ; Science and Technology Planning Project of Xuzhou City(KC16NX073 ; Natural Science Foundation of Jiangsu Province(BK20160217) ; Applied Basic Research Program of Qinghai Province(2014-ZJ-710) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; BN2015027) ; KC15N0013)
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS类目Biochemistry & Molecular Biology ; Biology
WOS记录号WOS:000433261800007
出版者IVYSPRING INT PUBL
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/13823
专题中国科学院西北高原生物研究所
通讯作者Fang, Xingtang
作者单位1.Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China
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Wang, Jian,Tan, Jiaoyan,Qi, Qi,et al. miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(7):760-774.
APA Wang, Jian.,Tan, Jiaoyan.,Qi, Qi.,Yang, Lingzhi.,Wang, Yanhong.,...&Fang, Xingtang.(2018).miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(7),760-774.
MLA Wang, Jian,et al."miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.7(2018):760-774.
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