A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences

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	A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences
	Yang, MH ; Zhang, DM ; Liu, JQ ; Zheng, JH
	2001-11-01
发表期刊	PLANTA MEDICA
卷号	67 期号:8 页码:784-786
文章类型	Letter
摘要	"Da-Huang" (Radix et Rhizoma Rhei, medicinal rhubarb), a famous and important Traditional Chinese Medicine, has often been confused with the adulterant species in the same genus, Rheum. Through sequencing the trnL (UAA)/trnF (GAA) regions of chloroplast DNA of thirteen species of Rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. A pair of PCR primers based on the sequences, was thus designed, which amplified a highly specific DNA fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized PCR condition.; "Da-Huang" (Radix et Rhizoma Rhei, medicinal rhubarb), a famous and important Traditional Chinese Medicine, has often been confused with the adulterant species in the same genus, Rheum. Through sequencing the trnL (UAA)/trnF (GAA) regions of chloroplast DNA of thirteen species of Rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of the medicinal species was found. A pair of PCR primers based on the sequences, was thus designed, which amplified a highly specific DNA fragment in medicinal rhubarb exclusively, and absent in the adulterants at all under an optimized PCR condition.
关键词	Dna Genome
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
学科领域	植物学
关键词[WOS]	DNA ; GENOME
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000172234800028
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1423
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Inst Bot, Lab Systemat & Evolut Bot, Beijing 100093, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Yang, MH,Zhang, DM,Liu, JQ,et al. A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences[J]. PLANTA MEDICA,2001,67(8):784-786.
APA	Yang, MH,Zhang, DM,Liu, JQ,&Zheng, JH.(2001).A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences.PLANTA MEDICA,67(8),784-786.
MLA	Yang, MH,et al."A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences".PLANTA MEDICA 67.8(2001):784-786.