Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H plus -ATPase and Na plus /H plus antiporter protein in salt-stressed callus from Carex moorcroftii

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	Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H plus -ATPase and Na plus /H plus antiporter protein in salt-stressed callus from Carex moorcroftii
	Li, Jisheng 1,2; Chen, Guichen 1; Wang, Xiaomin 2; Zhang, Yanli 2; Jia, Honglei 2; Bi, Yurong 1,2
	2011-03-01
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	141 期号:3 页码:239-250
文章类型	Article
摘要	Glucose-6-phosphate dehydrogenase (G6PDH) is important for the activation of plant resistance to environmental stresses, and ion homeostasis is the physiological foundation for living cells. In this study, we investigated G6PDH roles in modulating ion homeostasis under salt stress in Carex moorcroftii callus. G6PDH activity increased to its maximum in 100 mM NaCl treatment and decreased with further increased NaCl concentrations. K+/Na+ ratio in 100 mM NaCl treatment did not exhibit significant difference compared with the control; however, in 300 mM NaCl treatment, it decreased. Low-concentration NaCl (100 mM) stimulated plasma membrane (PM) H+-ATPase and NADPH oxidase activities as well as Na+/H+ antiporter protein expression, whereas high-concentration NaCl (300 mM) decreased their activity and expression. When G6PDH activity and expression were reduced by glycerol treatments, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein level and K+/Na+ ratio dramatically decreased. Simultaneously, NaCl-induced hydrogen peroxide (H(2)O(2)) accumulation was abolished. Exogenous application of H(2)O(2) increased G6PDH, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein expression and K+/Na+ ratio in the control and glycerol treatments. Diphenylene iodonium (DPI), the NADPH oxidase inhibitor, which counteracted NaCl-induced H(2)O(2) accumulation, decreased G6PDH, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein level and K+/Na+ ratio. Western blot result showed that G6PDH expression was stimulated by NaCl and H(2)O(2), and blocked by DPI. Taken together, G6PDH is involved in H(2)O(2) accumulation under salt stress. H(2)O(2), as a signal, upregulated PM H+-ATPase activity and Na+/H+ antiporter protein level, which subsequently resulted in the enhanced K+/Na+ ratio. G6PDH played a central role in the process.; Glucose-6-phosphate dehydrogenase (G6PDH) is important for the activation of plant resistance to environmental stresses, and ion homeostasis is the physiological foundation for living cells. In this study, we investigated G6PDH roles in modulating ion homeostasis under salt stress in Carex moorcroftii callus. G6PDH activity increased to its maximum in 100 mM NaCl treatment and decreased with further increased NaCl concentrations. K+/Na+ ratio in 100 mM NaCl treatment did not exhibit significant difference compared with the control; however, in 300 mM NaCl treatment, it decreased. Low-concentration NaCl (100 mM) stimulated plasma membrane (PM) H+-ATPase and NADPH oxidase activities as well as Na+/H+ antiporter protein expression, whereas high-concentration NaCl (300 mM) decreased their activity and expression. When G6PDH activity and expression were reduced by glycerol treatments, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein level and K+/Na+ ratio dramatically decreased. Simultaneously, NaCl-induced hydrogen peroxide (H(2)O(2)) accumulation was abolished. Exogenous application of H(2)O(2) increased G6PDH, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein expression and K+/Na+ ratio in the control and glycerol treatments. Diphenylene iodonium (DPI), the NADPH oxidase inhibitor, which counteracted NaCl-induced H(2)O(2) accumulation, decreased G6PDH, PM H+-ATPase and NADPH oxidase activities, Na+/H+ antiporter protein level and K+/Na+ ratio. Western blot result showed that G6PDH expression was stimulated by NaCl and H(2)O(2), and blocked by DPI. Taken together, G6PDH is involved in H(2)O(2) accumulation under salt stress. H(2)O(2), as a signal, upregulated PM H+-ATPase activity and Na+/H+ antiporter protein level, which subsequently resulted in the enhanced K+/Na+ ratio. G6PDH played a central role in the process.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	PUTATIVE NA+/H+ ANTIPORTER ; OXIDATIVE STRESS ; REACTIVE OXYGEN ; NITRIC-OXIDE ; POPULUS-EUPHRATICA ; ION HOMEOSTASIS ; ARABIDOPSIS-THALIANA ; SIGNAL-TRANSDUCTION ; GLUTATHIONE LEVELS ; TAXUS-CHINENSIS
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000287200300005
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1586
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Jisheng,Chen, Guichen,Wang, Xiaomin,et al. Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H plus -ATPase and Na plus /H plus antiporter protein in salt-stressed callus from Carex moorcroftii[J]. PHYSIOLOGIA PLANTARUM,2011,141(3):239-250.
APA	Li, Jisheng,Chen, Guichen,Wang, Xiaomin,Zhang, Yanli,Jia, Honglei,&Bi, Yurong.(2011).Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H plus -ATPase and Na plus /H plus antiporter protein in salt-stressed callus from Carex moorcroftii.PHYSIOLOGIA PLANTARUM,141(3),239-250.
MLA	Li, Jisheng,et al."Glucose-6-phosphate dehydrogenase-dependent hydrogen peroxide production is involved in the regulation of plasma membrane H plus -ATPase and Na plus /H plus antiporter protein in salt-stressed callus from Carex moorcroftii".PHYSIOLOGIA PLANTARUM 141.3(2011):239-250.