Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification

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	Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification
	Li Xiao-Yan 1; You Jin-Mao 1,2; Sun Zhi-Wei 1,3; Fu Yan-Yan 1; Qin Xue-Qin 1; Ding Ming-Zhou 1; Li Yu-Lin 2
	2010-07-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	38 期号:7 页码:917-923
文章类型	Article
摘要	By using 2-[2-(7H-dibenzo[a,g]carbazole-ethoxy)-ethyl chloroformate (DBCEC-Cl) as a sensitive pre-column derivatization reagent, a simple method for evaluating the chemical compositions of protein amino acids from the hydrolyzed cicada sample has been developed. Studies on derivatization conditions indicated that the labeling reaction proceeded rapidly and smoothly in the presence of a base catalyst ( pH 9.0) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 300 nm and an emission maximum at lambda(em) 395 nm. The separation of amino acid derivatives was carried out on a reversed phase Hypersil BDS-C(18) (200 mm x 4. 6 mm x 5 mu m) column in combination of a gradient elution. The complete baseline resolution for 20 amino acid derivatives was achieved within 65 min. Identification of amino acid derivatives was carried out by the online post-column ion trap/mass spectrometry with electrospray ion source (ESI source) in positive ion mode. Excellent linear response was observed, with coefficients of >0.9992. Fluorescent detection limits calculated from 25 pmol injection, at a signal-to-noise ratio of 3, were 2.6 - 24 fmol. The established method for the determination of amino acids from cicada sample was satisfactory.; By using 2-[2-(7H-dibenzo[a,g]carbazole-ethoxy)-ethyl chloroformate (DBCEC-Cl) as a sensitive pre-column derivatization reagent, a simple method for evaluating the chemical compositions of protein amino acids from the hydrolyzed cicada sample has been developed. Studies on derivatization conditions indicated that the labeling reaction proceeded rapidly and smoothly in the presence of a base catalyst ( pH 9.0) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 300 nm and an emission maximum at lambda(em) 395 nm. The separation of amino acid derivatives was carried out on a reversed phase Hypersil BDS-C(18) (200 mm x 4. 6 mm x 5 mu m) column in combination of a gradient elution. The complete baseline resolution for 20 amino acid derivatives was achieved within 65 min. Identification of amino acid derivatives was carried out by the online post-column ion trap/mass spectrometry with electrospray ion source (ESI source) in positive ion mode. Excellent linear response was observed, with coefficients of >0.9992. Fluorescent detection limits calculated from 25 pmol injection, at a signal-to-noise ratio of 3, were 2.6 - 24 fmol. The established method for the determination of amino acids from cicada sample was satisfactory.
关键词	High Performance Liquid Chromatography-ion Trap Mass Spectrometry Fluorescence Detection Pre-column Derivatization Amino Acids 2-[2-(7h-dibenzo[a g]Carbazol-7-yl)-ethoxy] Ethyl Chloroformate
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; HPLC ; PHTHALDIALDEHYDE ; SYSTEM
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000280677000001
引用统计
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1674
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li Xiao-Yan,You Jin-Mao,Sun Zhi-Wei,et al. Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(7):917-923.
APA	Li Xiao-Yan.,You Jin-Mao.,Sun Zhi-Wei.,Fu Yan-Yan.,Qin Xue-Qin.,...&Li Yu-Lin.(2010).Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(7),917-923.
MLA	Li Xiao-Yan,et al."Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38.7(2010):917-923.