Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent

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	Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent
	Zhang, Shijuan ; Yu, Zhaoqiang ; Hu, Na ; Sun, Yuanpeng ; Suo, Yourui ; You, Jinmao
	2014-05-01
发表期刊	FOOD CONTROL ; Zhang, SJ; Yu, ZQ; Hu, N; Sun, YP; Suo, YR; You, JM.Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent,FOOD CONTROL,2014,39():25
摘要	A new and sensitive pre-column derivatization method was developed for the analysis of melamine leached from tableware by high performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity was greatly enhanced by introducing 10-methyl-acridone-2-sulfonyl chloride (MASC) with excellent fluorescence property into the melamine molecule. Meanwhile, derivatization also greatly increased the hydrophobicity of melamine. Therefore, the common reversed phase column can be used for the HPLC analysis of highly hydrophilic melamine. The detection limit obtained by the proposed method was lower than 0.40 mu g/L. This is the first time that HPLC with fluorescence detection was applied to the analysis of melamine. The proposed method was successfully applied to the analysis of melamine leached from tableware. The results indicated that the leaching of melamine from tableware was obvious when hot water or milk was added. (C) 2013 Elsevier Ltd. All rights reserved.; A new and sensitive pre-column derivatization method was developed for the analysis of melamine leached from tableware by high performance liquid chromatography (HPLC) with fluorescence detection. The HPLC sensitivity was greatly enhanced by introducing 10-methyl-acridone-2-sulfonyl chloride (MASC) with excellent fluorescence property into the melamine molecule. Meanwhile, derivatization also greatly increased the hydrophobicity of melamine. Therefore, the common reversed phase column can be used for the HPLC analysis of highly hydrophilic melamine. The detection limit obtained by the proposed method was lower than 0.40 mu g/L. This is the first time that HPLC with fluorescence detection was applied to the analysis of melamine. The proposed method was successfully applied to the analysis of melamine leached from tableware. The results indicated that the leaching of melamine from tableware was obvious when hot water or milk was added. (C) 2013 Elsevier Ltd. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/17006
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Shijuan,Yu, Zhaoqiang,Hu, Na,et al. Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent[J]. FOOD CONTROL, Zhang, SJ; Yu, ZQ; Hu, N; Sun, YP; Suo, YR; You, JM.Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent,FOOD CONTROL,2014,39():25,2014.
APA	Zhang, Shijuan,Yu, Zhaoqiang,Hu, Na,Sun, Yuanpeng,Suo, Yourui,&You, Jinmao.(2014).Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent.FOOD CONTROL.
MLA	Zhang, Shijuan,et al."Sensitive determination of melamine leached from tableware by reversed phase high-performance liquid chromatography using 10-methyl-acridone-2-sulfonyl chloride as a pre-column fluorescent labeling reagent".FOOD CONTROL (2014).