Knowledge Management System of Northwest Institute of Plateau Biology, CAS
An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification | |
You, Jinmao1,2; Song, Cuihua1; Yan, Tan1; Sun, Zhiwei2,3; Li, Yulin2; Suo, Yourui2 | |
2010-01-18 | |
发表期刊 | ANALYTICA CHIMICA ACTA
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ISSN | 0003-2670 |
卷号 | 658期号:1页码:98-105 |
文章类型 | Article |
摘要 | An improved reagent named 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (DBCEC-Cl) for the determination of aliphatic amines by high-performance liquid chromatography (HPLC) with fluorescence detection and post-column online atmospheric chemical ionization-mass spectrometry (APCI-MS) identification has been developed. DBCEC-Cl could easily and quickly label aliphatic amines. Derivatives were stable enough to be efficiently analyzed by HPLC and showed an intense protonated molecular ion corresponding m/z [M+H](+) under APCI-MS in positive-ion mode. The ratios for fluorescence responses were I(DBCEC-amine)/I(BCE-amine) = 1.02-1.60; I(DBCEC-amine)/I(BCEOC-amine) = 1.30-2.57: and I(DBCEC-amine)/I(FMOC-amine) = 2.20-4.12 (here, I was relative fluorescence intensity). The ratios for MS responses were IC(DBCEC-amine)/IC(BCEC-amine) = 4.16-29.31 and IC(DBCEC-amine)/IC(BCEOC-amine) = 1.23-2.47 (Here, IC: APCI-MS ion current intensity). Detection limits calculated from 0.0244 pmol injection, at a signal-to-noise ratio of 3, were 0.3-3.0 fmol. The relative standard deviations for within-day determination (n=6) were 0.045-0.081% for retention time and 0.86-1.03% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amine levels were <3.64% and 4.67%, respectively. The mean recoveries ranged from 96.9% to 104.7% with their standard deviations in the range of 1.80-2.70 (RSDs%). Excellent linear responses were observed with coefficients of >0.9991. (C) 2009 Elsevier B.V. All rights reserved.; An improved reagent named 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (DBCEC-Cl) for the determination of aliphatic amines by high-performance liquid chromatography (HPLC) with fluorescence detection and post-column online atmospheric chemical ionization-mass spectrometry (APCI-MS) identification has been developed. DBCEC-Cl could easily and quickly label aliphatic amines. Derivatives were stable enough to be efficiently analyzed by HPLC and showed an intense protonated molecular ion corresponding m/z [M+H](+) under APCI-MS in positive-ion mode. The ratios for fluorescence responses were I(DBCEC-amine)/I(BCE-amine) = 1.02-1.60; I(DBCEC-amine)/I(BCEOC-amine) = 1.30-2.57: and I(DBCEC-amine)/I(FMOC-amine) = 2.20-4.12 (here, I was relative fluorescence intensity). The ratios for MS responses were IC(DBCEC-amine)/IC(BCEC-amine) = 4.16-29.31 and IC(DBCEC-amine)/IC(BCEOC-amine) = 1.23-2.47 (Here, IC: APCI-MS ion current intensity). Detection limits calculated from 0.0244 pmol injection, at a signal-to-noise ratio of 3, were 0.3-3.0 fmol. The relative standard deviations for within-day determination (n=6) were 0.045-0.081% for retention time and 0.86-1.03% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amine levels were <3.64% and 4.67%, respectively. The mean recoveries ranged from 96.9% to 104.7% with their standard deviations in the range of 1.80-2.70 (RSDs%). Excellent linear responses were observed with coefficients of >0.9991. (C) 2009 Elsevier B.V. All rights reserved. |
关键词 | Derivatization Fluorescence Amines 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate Atmospheric Chemical Ionization-mass Spectrometry |
WOS标题词 | Science & Technology ; Physical Sciences |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; VOLATILE N-NITROSAMINES ; DERIVATIZING REAGENT ; BIOGENIC-AMINES ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; PRECOLUMN DERIVATIZATION ; LABELING REAGENT ; ENZYMATIC METHOD ; WATER SAMPLES ; CHLOROFORMATE |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000273847500014 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1754 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | You, Jinmao,Song, Cuihua,Yan, Tan,et al. An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification[J]. ANALYTICA CHIMICA ACTA,2010,658(1):98-105. |
APA | You, Jinmao,Song, Cuihua,Yan, Tan,Sun, Zhiwei,Li, Yulin,&Suo, Yourui.(2010).An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification.ANALYTICA CHIMICA ACTA,658(1),98-105. |
MLA | You, Jinmao,et al."An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification".ANALYTICA CHIMICA ACTA 658.1(2010):98-105. |
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