Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments

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	Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments
	Cao, Yi-Bin ; Chen, Xue-Qun 1; Wang, Shen ; Chen, Xiao-Cheng 2; Wang, Yu-Xiang 3; Chang, J. P.4; Du, Ji-Zeng
	2009-05-01
发表期刊	GENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN	0016-6480
卷号	161 期号:3 页码:400-406
文章类型	Article
摘要	Here, we report the cloning and characterization of growth hormone (GH), insulin-like growth factor-I (IGF-I) and IGF-II from naked carp (Gymnocypris przewalskii), a native teleost fish of Lake Qinghai in the Qinghai-Tibet Plateau of China. The GH of naked carp encodes for a predicted amino acid sequence showing identities of 63%, 63%, 91% and 94% with cherry salmon, rainbow trout, zebrafish and grass carp, respectively, Compared to common carp and goldfish, evolutionary analysis showed that genome duplication has had less influence on the relaxation of purifying selection in the evolution of naked carp GH. Sequence analysis of naked carp IGF-I (ncIGF-I) and ncIGF-II showed a high degree of homology with known fish IGF-I and IGF-II. To investigate effects of salinity and ionic composition of the aquatic environment on the GH-IGF axis in naked carp, male fish held in river water were assigned randomly to 4 groups: RW (river-water), RW + Na (NaCl in RW), RW + Mg (MgCl(2) in RW) and LW (lake-water) groups. The concentrations of Na(+) in RW + Na and Mg(2+) in RW + Mg were equal to the concentrations of these ions in lake-water. After 2 days of exposure, the plasma IGF-I levels in the RW + Na and LW groups were significantly higher than the control group (RW), and the plasma GH levels of the LW group were also significantly higher than the RW group. The somatostatin (SS) levels in the hypothalamus significantly increased in the RW + Na group. After 5 days of exposure, these hormone levels did not differ significantly among groups. These results indicate that while the plasma GH and IGF-I levels are osmosensitive, the absence of a change in GH secretion in RW + Na might be partly due to a transiently increased release of hypothalamic SS induced by the stress of neutral-saline water. This is the first report of a salinity-induced increase of GH-IGF-I circulating levels in Cypriniformes. (C) 2009 Elsevier Inc. All rights reserved.; Here, we report the cloning and characterization of growth hormone (GH), insulin-like growth factor-I (IGF-I) and IGF-II from naked carp (Gymnocypris przewalskii), a native teleost fish of Lake Qinghai in the Qinghai-Tibet Plateau of China. The GH of naked carp encodes for a predicted amino acid sequence showing identities of 63%, 63%, 91% and 94% with cherry salmon, rainbow trout, zebrafish and grass carp, respectively, Compared to common carp and goldfish, evolutionary analysis showed that genome duplication has had less influence on the relaxation of purifying selection in the evolution of naked carp GH. Sequence analysis of naked carp IGF-I (ncIGF-I) and ncIGF-II showed a high degree of homology with known fish IGF-I and IGF-II. To investigate effects of salinity and ionic composition of the aquatic environment on the GH-IGF axis in naked carp, male fish held in river water were assigned randomly to 4 groups: RW (river-water), RW + Na (NaCl in RW), RW + Mg (MgCl(2) in RW) and LW (lake-water) groups. The concentrations of Na(+) in RW + Na and Mg(2+) in RW + Mg were equal to the concentrations of these ions in lake-water. After 2 days of exposure, the plasma IGF-I levels in the RW + Na and LW groups were significantly higher than the control group (RW), and the plasma GH levels of the LW group were also significantly higher than the RW group. The somatostatin (SS) levels in the hypothalamus significantly increased in the RW + Na group. After 5 days of exposure, these hormone levels did not differ significantly among groups. These results indicate that while the plasma GH and IGF-I levels are osmosensitive, the absence of a change in GH secretion in RW + Na might be partly due to a transiently increased release of hypothalamic SS induced by the stress of neutral-saline water. This is the first report of a salinity-induced increase of GH-IGF-I circulating levels in Cypriniformes. (C) 2009 Elsevier Inc. All rights reserved.
关键词	Naked Carp Qinghai Lake Gh Igf-i Igf-ii Salinity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	CATFISH ICTALURUS-PUNCTATUS ; EEL ANGUILLA-ANGUILLA ; FACTOR-I AXIS ; MESSENGER-RNA ; IGF-I ; OREOCHROMIS-MOSSAMBICUS ; CARASSIUS-AURATUS ; RAINBOW-TROUT ; FISH ; PROLACTIN
收录类别	SCI
语种	英语
WOS研究方向	Endocrinology & Metabolism
WOS类目	Endocrinology & Metabolism
WOS记录号	WOS:000265348500013
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1882
专题	中国科学院西北高原生物研究所
作者单位	1.Zhejiang Univ, Div Neurobiol & Physiol, Coll Life Sci, Sch Med, Hangzhou 310058, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 3.Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada 4.Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
推荐引用方式 GB/T 7714	Cao, Yi-Bin,Chen, Xue-Qun,Wang, Shen,et al. Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2009,161(3):400-406.
APA	Cao, Yi-Bin.,Chen, Xue-Qun.,Wang, Shen.,Chen, Xiao-Cheng.,Wang, Yu-Xiang.,...&Du, Ji-Zeng.(2009).Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments.GENERAL AND COMPARATIVE ENDOCRINOLOGY,161(3),400-406.
MLA	Cao, Yi-Bin,et al."Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments".GENERAL AND COMPARATIVE ENDOCRINOLOGY 161.3(2009):400-406.