Effects of emodin on the gene expression profiling of human breast carcinoma cells

NWIPB OpenIR

	Effects of emodin on the gene expression profiling of human breast carcinoma cells
	Huang, Zhiwei 2,3; Chen, Guichen 3; Shi, Ping 1,3
	2009
发表期刊	CANCER DETECTION AND PREVENTION
ISSN	0361-090X
卷号	32 期号:4 页码:286-291
文章类型	Article
摘要	Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd.; Background: The mechanism of emodin-mediated cell apoptosis has been investigated extensively in many types of human cancer cells. Our previous study demonstrated that emodin induced apoptosis through the decrease of Bcl-2/Bax ratio and the increase of cytoplasm cytochrome c concentration in human breast cancer BCap-37 cells. However, emodin's reaction to breast cancer cells remains elusive. Materials and methods: An apoptosis-associated cDNA microarray comprised of 458 known genes, namely, death receptors, calpains, death kinases, granzymes, DNA fragmentation proteins, caspases and Bcl-2 family, was used to determine the impact of emodin in breast cancer BCap-37 cells. Furthermore, the candidate emodin target genes were further evaluated via real-time quantitative PCR and Western blot analysis. Results: We found that gene expression profiling in human breast cancer BCap-37 cells was altered when exposed to emodin. Thirty of the unique genes that were either induced or repressed in response to emodin-induced apoptosis were also identified. A follow-up study characterized p53, emodin-induced gene, IGF-2, and emodin-repressed gene, and the downstream proteins were also seen as possible molecular targets of emodin. Conclusion: Data from this study provide novel evidence that emodin induces gene expression profiling changes, but has no effects on caspases. In addition, the p53 pathway may cooperate with the IGF-2 pathway, resulting in an emodin-induced apoptosis through disruption of the mitochondrial signaling pathway in BCap-37 cells. Published by Elsevier Ltd.
关键词	Emodin Human Breast Cancer Bcap-37 Cells Apoptosis Cdna Microarray Mitochondrial Signaling Pathway Igf-2
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	GROWTH-FACTOR-II ; MITOCHONDRIAL SIGNALING PATHWAY ; COP9 SIGNALOSOME ; INDUCED APOPTOSIS ; GASTRIC-CANCER ; TUMOR-CELLS ; KAPPA-B ; P53 ; SUPPRESSION ; ACTIVATION
收录类别	SCI
语种	英语
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000264618800002
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1918
专题	中国科学院西北高原生物研究所
作者单位	1.Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Unit 54, Houston, TX 77030 USA 2.Baylor Coll Med, Houston, TX 77030 USA 3.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Huang, Zhiwei,Chen, Guichen,Shi, Ping. Effects of emodin on the gene expression profiling of human breast carcinoma cells[J]. CANCER DETECTION AND PREVENTION,2009,32(4):286-291.
APA	Huang, Zhiwei,Chen, Guichen,&Shi, Ping.(2009).Effects of emodin on the gene expression profiling of human breast carcinoma cells.CANCER DETECTION AND PREVENTION,32(4),286-291.
MLA	Huang, Zhiwei,et al."Effects of emodin on the gene expression profiling of human breast carcinoma cells".CANCER DETECTION AND PREVENTION 32.4(2009):286-291.