Applica tion of 2-[ 2-( 7H-D ibenzo[ a, g] –carbazol-7-yl) 2ethoxyl] ethyl Chloroforma te a s A Pre-column Der iva tiza tion Reagent for Determ ina tion of Am ino Ac ids from Hydrolyzed C icada by High Performance L iqu id Chroma tography with Fluorescence Detection and Ma ss Spectrometr ic Identif ica tion

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	Applica tion of 2-[ 2-( 7H-D ibenzo[ a, g] –carbazol-7-yl) 2ethoxyl] ethyl Chloroforma te a s A Pre-column Der iva tiza tion Reagent for Determ ina tion of Am ino Ac ids from Hydrolyzed C icada by High Performance L iqu id Chroma tography with Fluorescence Detection and Ma ss Spectrometr ic Identif ica tion
	Jinmao You
	2010
发表期刊	Chinese Journal of Analytical Chemistry
ISSN	0253-3820
卷号	38 期号:7 页码:917-923
其他摘要	Based on the use of 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as a sensitive pre-column derivatization reagent, a simple method for evaluating the chemical compositions of the hydrolyzed protein from the cicada nymphs has been developed. Studies on derivatization conditions indicated that the labeling reaction proceeded rapidly and smoothly in the presence of a base catalyst (pH 9.0) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at ？ex of 300 nm and an emission maximum at ？em of 395 nm. The separation of amino acid derivatives was carried out on a reversed-phase Hypersil BDS-C18 (200 mm × 4.6 mm, 5 μm) column in combination with a gradient elution. The complete baseline resolution for 20 amino acid derivatives was achieved within 65 minutes. Identification of amino acid derivatives was carried out by the on-line ion trap/mass spectrometry with electrospray ion source in positive-ion mode. Excellent linear response was observed with coefficients > 0.9992. Fluorescent detection limits calculated from 25 fmol injection (at a signal-to-noise ratio of 3) were 2.60–24.3 fmol. The established method for the determination of amino acids from the hydrolyzed cicada sample was satisfactory.
收录类别	SCI
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1974
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jinmao You. Applica tion of 2-[ 2-( 7H-D ibenzo[ a, g] –carbazol-7-yl) 2ethoxyl] ethyl Chloroforma te a s A Pre-column Der iva tiza tion Reagent for Determ ina tion of Am ino Ac ids from Hydrolyzed C icada by High Performance L iqu id Chroma tography with Fluorescence Detection and Ma ss Spectrometr ic Identif ica tion[J]. Chinese Journal of Analytical Chemistry,2010,38(7):917-923.
APA	Jinmao You.(2010).Applica tion of 2-[ 2-( 7H-D ibenzo[ a, g] –carbazol-7-yl) 2ethoxyl] ethyl Chloroforma te a s A Pre-column Der iva tiza tion Reagent for Determ ina tion of Am ino Ac ids from Hydrolyzed C icada by High Performance L iqu id Chroma tography with Fluorescence Detection and Ma ss Spectrometr ic Identif ica tion.Chinese Journal of Analytical Chemistry,38(7),917-923.
MLA	Jinmao You."Applica tion of 2-[ 2-( 7H-D ibenzo[ a, g] –carbazol-7-yl) 2ethoxyl] ethyl Chloroforma te a s A Pre-column Der iva tiza tion Reagent for Determ ina tion of Am ino Ac ids from Hydrolyzed C icada by High Performance L iqu id Chroma tography with Fluorescence Detection and Ma ss Spectrometr ic Identif ica tion".Chinese Journal of Analytical Chemistry 38.7(2010):917-923.