One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography

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	One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography
	Shi, Junyou ; Li, Guoliang ; Wang, Honglun ; Zheng, Jie ; Suo, Yourui ; You, Jinmao ; Liu, Yongjun
	2011-09-01
发表期刊	PHYTOCHEMICAL ANALYSIS ; Shi, JY; Li, GL; Wang, HL; Zheng, J; Suo, YR; You, JM; Liu, YJ.One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2011,22(5):450-454
摘要	Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. Objective - To develop an efficient method for the preparative isolation and purification of flavonoids from the leaves of Poacynum hendersonii by high-speed counter-current chromatography (HSCCC). Methodology - Powdered Poacynum hendersonii lead was extracted three times with 75% ethanol at 60 degrees C for 3 h. The distribution constant (K(D)) was measured to select an optimal two-phase solvent system for HSCCC separation. The purities of the target compounds were tested using HPLC and their structures were identified by (1)H-NMR and (13)C-NMR. Results - Using a two-phase solvent system composed of n-butanol-petroleum ether-0.5% acetic acid (5:3:5, v/v), three main flavonoids, i.e. isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside, were separated from 240 mg crude sample in a one-step separation by using HSCCC method. After further purification with a Sepdex-LH20 column, 5.7 mg isoquercitrin (LC purity 98.72%), 4.9 mg quercetin-3-O-sophoroside (LC purity 99.06%) and 7.4 mg quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside (LC purity 99.31%) were obtained, respectively. Conclusion - The optimised high-speed counter-current chromatography method is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii. Copyright (C) 2011 John Wiley & Sons, Ltd.; Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20432
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Shi, Junyou,Li, Guoliang,Wang, Honglun,et al. One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS, Shi, JY; Li, GL; Wang, HL; Zheng, J; Suo, YR; You, JM; Liu, YJ.One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography,PHYTOCHEMICAL ANALYSIS,2011,22(5):450-454,2011.
APA	Shi, Junyou.,Li, Guoliang.,Wang, Honglun.,Zheng, Jie.,Suo, Yourui.,...&Liu, Yongjun.(2011).One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS.
MLA	Shi, Junyou,et al."One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS (2011).