Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers

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	Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers
	Xie, Xiaolong ; Hu, Yanping ; Wang, Li ; Yang, Jian ; Li, Yi ; Peng, Min
	2009-09-15
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY ; Xie, XL; Hu, YP; Wang, L; Yang, J; Li, Y; Peng, M.Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers,AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(18):4490-4494
摘要	Inter simple sequence repeat (ISSR) markers were used to detect the genetic diversity in three morphological types of cultivated Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao discovered in Gansu province of China. Eight primers used for analysis generated 165 scorable bands of which 162 (98.18%) were polymorphic. The percentage polymorphic bands (PPB), Shannon's information index (I) and Nei's gene diversity (h) for 3 types ranged from 59.39%, 0.2912, 0.1901 to 78.18%, 0.3411, 0.2238 respectively, indicating each type had a high genetic diversity. Analysis of molecular variance (AMOVA) showed 90.41% of the total genetic diversity existed within the 3 morphological types, whereas only 9.59% occurred among them. The UPGMA tree based on SM similarity coefficients implied that the 3 types had genetically differentiated though not obviously. The high genetic diversity suggested that each type had a potential to develop new strains or cultivars and ISSR was a potentially useful tool in the process of selective breeding of A. membranaceus var. mongholicus.; Inter simple sequence repeat (ISSR) markers were used to detect the genetic diversity in three morphological types of cultivated Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao discovered in Gansu province of China. Eight primers used for analysis generated 165 scorable bands of which 162 (98.18%) were polymorphic. The percentage polymorphic bands (PPB), Shannon's information index (I) and Nei's gene diversity (h) for 3 types ranged from 59.39%, 0.2912, 0.1901 to 78.18%, 0.3411, 0.2238 respectively, indicating each type had a high genetic diversity. Analysis of molecular variance (AMOVA) showed 90.41% of the total genetic diversity existed within the 3 morphological types, whereas only 9.59% occurred among them. The UPGMA tree based on SM similarity coefficients implied that the 3 types had genetically differentiated though not obviously. The high genetic diversity suggested that each type had a potential to develop new strains or cultivars and ISSR was a potentially useful tool in the process of selective breeding of A. membranaceus var. mongholicus.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20589
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xie, Xiaolong,Hu, Yanping,Wang, Li,et al. Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, Xie, XL; Hu, YP; Wang, L; Yang, J; Li, Y; Peng, M.Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers,AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(18):4490-4494,2009.
APA	Xie, Xiaolong,Hu, Yanping,Wang, Li,Yang, Jian,Li, Yi,&Peng, Min.(2009).Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers.AFRICAN JOURNAL OF BIOTECHNOLOGY.
MLA	Xie, Xiaolong,et al."Genetic diversity in three morphological types of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao as revealed by inter simple sequence repeat markers".AFRICAN JOURNAL OF BIOTECHNOLOGY (2009).