Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity

NWIPB OpenIR

	Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity
	Li Guoliang 1,2; Shi Junyou 1,2; Suo Yourui 1; Sun Zhiwei 1,2; Xia Lian 1,2; Zheng Jie 1,2; You Jinmao 1; Liu Yongjun 1
	2011-05-01
发表期刊	LWT-FOOD SCIENCE AND TECHNOLOGY
ISSN	0023-6438
卷号	44 期号:4 页码:1172-1178
文章类型	Article
摘要	The extraction parameters for oil extraction from Lycium barbarum seed including extraction pressure, temperature and time were optimized using an orthogonal test design. The optimum conditions for supercritical CO2 extraction were as follows: extraction pressure, 30 MPa; extraction temperature, 45 _C; dynamic extraction time, 60 min; CO2 flow, 25 kg/h. The oil yield under the conditions proposed was 19.28 g/100 g. The effect of cell wall breakage pretreatment was investigated by supercritical CO2 rapid depressurization, and results indicated this pretreatment could result in a rapid and efficient extraction. A sensitive fluorescent reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl 4-methylbenzenesulfonate (BCETS) was utilized as pre-column labeling regent to determine fatty acids (FA) from Lycium barbarum seed oils obtained by different extraction methods. The main FA were: C18:2, C18:1, C16, C20:6, C18:3, and C20. The oil from L. barbarum seed exhibited excellent antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl （DPPH）radical scavenging assay and b-carotene bleaching test, and its antioxidant activity compared well with the references ascorbic acid and a- tocopherol.; The extraction parameters for oil extraction from Lycium barbarum seed including extraction pressure, temperature and time were optimized using an orthogonal test design. The optimum conditions for supercritical CO2 extraction were as follows: extraction pressure, 30 MPa; extraction temperature, 45 degrees C; dynamic extraction time, 60 min; CO2 flow, 25 kg/h. The oil yield under the conditions proposed was 19.28 g/100 g. The effect of cell wall breakage pretreatment was investigated by supercritical CO2 rapid depressurization, and results indicated this pretreatment could result in a rapid and efficient extraction. A sensitive fluorescent reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl 4-methylbenzenesulfonate (BCETS) was utilized as pre-column labeling regent to determine fatty acids (FA) from Lycium barbarum seed oils obtained by different extraction methods. The main FA were: C18:2, C18:1, C16, C20:6, C18:3, and C20. The oil from L barbarum seed exhibited excellent antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and beta-carotene bleaching test, and its antioxidant activity compared well with the references ascorbic acid and alpha-tocopherol. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Lycium Barbarum Supercritical Co2 Seed Oil Fatty Acids Antioxidant Activity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	FATTY-ACIDS ; CARBON-DIOXIDE ; FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; FLUID EXTRACTION ; OXIDATIVE STRESS ; POLYSACCHARIDES ; SEPARATION
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000287641800051
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/2234
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li Guoliang,Shi Junyou,Suo Yourui,et al. Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2011,44(4):1172-1178.
APA	Li Guoliang.,Shi Junyou.,Suo Yourui.,Sun Zhiwei.,Xia Lian.,...&Liu Yongjun.(2011).Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity.LWT-FOOD SCIENCE AND TECHNOLOGY,44(4),1172-1178.
MLA	Li Guoliang,et al."Supercritical CO2 cell breaking extraction of Lycium barbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity".LWT-FOOD SCIENCE AND TECHNOLOGY 44.4(2011):1172-1178.