Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)

NWIPB OpenIR

	Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)
	Li, Zhong-Hu ; Wen, Hong-Yan ; Chen, Jian ; Wu, Gui-Li ; Wang, Yu-Jin
	2009-06-01
发表期刊	CONSERVATION GENETICS ; Li, ZH; Wen, HY; Chen, J; Wu, GL; Wang, YJ.Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae),CONSERVATION GENETICS,2009,10(3):747-749
摘要	Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species. These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.; Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species. These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/30802
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,et al. Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)[J]. CONSERVATION GENETICS, Li, ZH; Wen, HY; Chen, J; Wu, GL; Wang, YJ.Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae),CONSERVATION GENETICS,2009,10(3):747-749,2009.
APA	Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae).CONSERVATION GENETICS.
MLA	Li, Zhong-Hu,et al."Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)".CONSERVATION GENETICS (2009).