|Other Abstract||Meconopsis quintuplinervia Regel, belong to the species from the genus Meconopsis of Papaveraceae, were Perennial herb distributing in Qinghai, Tibet, Shaanxi, Gansu and Sichuan in P R China. As a extensive and important Tibetan medicine, the whole plant or flower can be use in medicine, with heat-clearing and detoxifying, diuretic, anti-inflammatory effectiveness, analgesic, such as the efficacy for the treatment of pneumonia, hepatitis, headache, edema, skin diseases and other diseases. Currently, most studies of it chemical constituents were focused on extraction and separation of the active ingredient in vivo, alkaloids, flavonoids, volatile oils, furans and other chemical constituents have been isolated from the medicinal material. However, the content of flavonoids in herbs and eco-environmental factors on their chemical composition were not involved in.
In this paper, throughout the ecological environment of the Qinghai-Tibet Plateau, an in-depth and wide range sample surveys were developed in the main distribution area of Qinghai area. With the whole plant of Meconopsis quintuplinervia as the study object, using UV spectrophotometry and HPLC accurate analysis the total alkaloids and luteolin, quercetin, kaempferol and isorhamnetin content quantity, also to calculate the total content of flavonoids. Soil nutrient content and PH value of sampling sites were determined by agricultural chemical method. Based on the experimental analysis, we explore the effects of ecological environment to chemical composition by statistical methods, and give a preliminary comparative analysis of the quality in different origin resources. The main findings and conclusions are summarized as follows:
1. Total alkaloid content between the samples was significantly different; flavonoids could be dived into two categories: flavanone (luteolin) and flavanone alcohols (quercetin, kaempferol and isorhamnetin), luteolin and quercetin occupied most in four flavonoids. The synergies were existented between three alcohols flavanone components.
2. Elevation, longitude and latitude were integrated embodiment of various ecological factors. The effects of latitude to total alkaloids and luteolin were significant, also elevation to luteolin.
3. Soil sample analysis results show that，when the soil rich in nitrogen, phosphorus, potassium and organic matter content，the contents of total alkaloid and quercetin, kaempferol and isorhamnetin with a high level in herbs, but the content of luteolin with a low level; and when soil with a high pH value, contents of total alkaloid and luteolin increase but the other three flavanone alcohols contents decrease. This may be related to internal medicine itself metabolism process.
4. Light, temperature and precipitation and other meteorological factors have a significant impact on the chemical composition too. In a certain extent, with the increase of sunshine hours, total alkaloids and total flavonoids ingredients increased, luteolin decreased. Total alkaloids and total flavonoids in herbs with a high level in low-temperature and humid weather conditions, and luteolin with a high level under high temperature and drought, but this may be also by low temperature and drought stress caused by the adversity.
5. The chemical constituents of herb between every origin are different. From the integrated quality analysis, counties of Dari and Maqin with a high level of total alkaloids, highest quality of flavonoids and the various components contained are the best integrated groups; Tongren and Xunhua both have the highest total alkaloid content, but low content of flavonoids, belong to high-quality alkaloids groups; Qilian and Minle of the highest luteolin content with other components in the low side, are high quality luteolin groups; Meconopsis quintuplinervia in other parts belonging to groups had no significant. in determination components.|