NWIPB OpenIR
Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions
Chen, Wen-Jie1; Fan, Xing1; Zhang, Bo2; Liu, Bao-Long2; Yan, Ze-Hong1; Zhang, Lian-Quan1; Yuan, Zhong-Wei1; Zheng, You-Liang1; Zhang, Huai-Gang2; Liu, Deng-Cai1,2
2012-12-01
发表期刊GENETIC RESOURCES AND CROP EVOLUTION
ISSN0925-9864
卷号59期号:8页码:1649-1657
文章类型Article
摘要A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx3.1(t) and 1Dy11*(t) were revealed and characterized from Aegilops tauschii Coss. subspecies tauschii accession AS60. SDS-PAGE band of 1Dx3.1(t) was between those of 1Dx2 and 1Dx3, while 1Dy11*(t) was between 1Dy11 and 1Dy12. The lengths of 1Dx3.1 (t) and 1Dy11* (t) were 2,514 bp and 1,968 bp, encoding 836 and 654 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. Network analysis indicated that 1Dx3.1 (t) together with other five rare alleles only detected in Asia common wheat populations represented the ancestral sequences in Glu-D1 locus. Neighbor-joining tree analysis of previously cloned x-type and y-type alleles in the Glu-D1 locus supported the hypothesis that more than one Ae. tauschii genotypes were involved in the origin of hexaploid wheat and that different Ae. tauschii accessions contributed the D genome to common wheat and Ae. cylindrical Host, respectively. An Ae. tauschii accession with 1Dx3.1 (t) or a closely related allele probably have involved in the origin of common wheat. Since accession AS60 used in this study belonged to typical ssp. tauschii, present results suggested the possibility that ssp. tauschii was involved in the evolution of common wheat.; A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx3.1(t) and 1Dy11*(t) were revealed and characterized from Aegilops tauschii Coss. subspecies tauschii accession AS60. SDS-PAGE band of 1Dx3.1(t) was between those of 1Dx2 and 1Dx3, while 1Dy11*(t) was between 1Dy11 and 1Dy12. The lengths of 1Dx3.1 (t) and 1Dy11* (t) were 2,514 bp and 1,968 bp, encoding 836 and 654 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. Network analysis indicated that 1Dx3.1 (t) together with other five rare alleles only detected in Asia common wheat populations represented the ancestral sequences in Glu-D1 locus. Neighbor-joining tree analysis of previously cloned x-type and y-type alleles in the Glu-D1 locus supported the hypothesis that more than one Ae. tauschii genotypes were involved in the origin of hexaploid wheat and that different Ae. tauschii accessions contributed the D genome to common wheat and Ae. cylindrical Host, respectively. An Ae. tauschii accession with 1Dx3.1 (t) or a closely related allele probably have involved in the origin of common wheat. Since accession AS60 used in this study belonged to typical ssp. tauschii, present results suggested the possibility that ssp. tauschii was involved in the evolution of common wheat.
关键词Aegilops Tauschii Bacterial Expression Hmw-gs Phylogenetic Analysis
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]MOLECULAR-WEIGHT SUBUNITS ; BREAD-MAKING QUALITY ; D-GENOME DONOR ; HEXAPLOID WHEAT ; TRITICUM-TAUSCHII ; ALLELIC VARIATION ; SEQUENCE DATA ; IDENTIFICATION ; AESTIVUM ; PROTEIN
收录类别SCI
语种英语
WOS研究方向Agriculture ; Plant Sciences
WOS类目Agronomy ; Plant Sciences
WOS记录号WOS:000311311700005
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/3578
专题中国科学院西北高原生物研究所
作者单位1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China
2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
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GB/T 7714
Chen, Wen-Jie,Fan, Xing,Zhang, Bo,et al. Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions[J]. GENETIC RESOURCES AND CROP EVOLUTION,2012,59(8):1649-1657.
APA Chen, Wen-Jie.,Fan, Xing.,Zhang, Bo.,Liu, Bao-Long.,Yan, Ze-Hong.,...&Liu, Deng-Cai.(2012).Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions.GENETIC RESOURCES AND CROP EVOLUTION,59(8),1649-1657.
MLA Chen, Wen-Jie,et al."Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions".GENETIC RESOURCES AND CROP EVOLUTION 59.8(2012):1649-1657.
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